Lysosomal changes caused by nicotine exposure in human epithelial tongue cells.

IF 2.9 3区 医学 Q2 TOXICOLOGY
S Uvsløkk, T Tanner, R Becher, H Valen, J T Samuelsen
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引用次数: 0

Abstract

Nicotine is an addictive substance and has been associated with several harmful effects on health. Many previous studies have focused on the receptor-mediated mechanisms of nicotine. However, non-receptor mediated effects of nicotine, such as effects on the lysosomes have also been reported. Well-functioning lysosomes are essential for cellular degradation pathways like the autophagic, phagocytic, and endocytic pathways. This study aimed to investigate nicotine's direct effect on lysosomes and lysosome-dependent activities in vitro. Cells from the immortalized human epithelial tongue cell line PE/CA-PJ49 were exposed to nicotine (5mM) alone or in combination with bafilomycin A1 (10nM; an inhibitor of lysosomal activity). Cell viability was measured using the MTT assay. Morphological changes were studied in a phase contrast microscope. Lysosomal activity was measured using flow cytometry and western blotting was used to quantify selected autophagy-related proteins. Only nicotine in combination with bafilomycin A1 resulted in decreased cell viability. However, morphological changes (vacuolization) were only observed in the cells exposed to nicotine. Apart from control, all exposures decreased lysosomal activity and increased the levels of the autophagy-related proteins p62/SQSTM1 and LC3-II. In conclusion, nicotine caused cellular vacuolization, reduced lysosomal activity, and increased levels of autophagy-related proteins indicating impaired autophagic flux.

尼古丁暴露对人舌上皮细胞溶酶体的影响。
尼古丁是一种令人上瘾的物质,对健康有几种有害影响。以前的许多研究都集中在尼古丁的受体介导机制上。然而,尼古丁的非受体介导效应,如对溶酶体的影响也有报道。功能良好的溶酶体是细胞降解途径如自噬、吞噬和内吞途径所必需的。本研究旨在探讨尼古丁对体外溶酶体及其依赖活性的直接影响。将永活人上皮舌细胞系PE/CA-PJ49细胞单独暴露于尼古丁(5mM)或联合巴菲霉素A1 (10nM;溶酶体活性的抑制剂)。采用MTT法测定细胞活力。在相差显微镜下观察其形态学变化。用流式细胞术测定溶酶体活性,用western blotting定量选定的自噬相关蛋白。只有尼古丁联合巴非霉素A1导致细胞活力下降。然而,形态学变化(空泡化)仅在暴露于尼古丁的细胞中观察到。除对照组外,所有暴露均降低溶酶体活性,增加自噬相关蛋白p62/SQSTM1和LC3-II的水平。总之,尼古丁导致细胞空泡化,溶酶体活性降低,自噬相关蛋白水平升高,表明自噬通量受损。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Toxicology letters
Toxicology letters 医学-毒理学
CiteScore
7.10
自引率
2.90%
发文量
897
审稿时长
33 days
期刊介绍: An international journal for the rapid publication of novel reports on a range of aspects of toxicology, especially mechanisms of toxicity.
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