SRSF3 Inhibits High Glucose and Palmitic Acid-Induced Cardiomyocyte Apoptosis Through the PI3K/AKT and NF-κB Pathways

Abstract

Diabetic cardiomyopathy (DCM) has emerged as a leading cause of mortality among elderly patients with diabetes mellitus (DM). Currently, the pathogenic mechanisms underlying diabetic cardiomyopathy remain elusive. In the present study, we treated with H9C2 cells high glucose (Glu) and palmitic acid (PA) to establish an in vitro model, followed by proteomic profiling. The proteomic analysis revealed that high glucose and palmitic acid levels downregulated the expression of SRSF3. Cell viability was assessed using the CCK-8 assay, whereas flow cytometry and western blot analysis were utilized to analyze cellular apoptosis. Quantitative reverse transcriptase–polymerase chain reaction (RT-PCR) was employed to examine the expression of SRSF3 and western blot analysis was conducted to assess the levels of Bax, Bcl-2, and proteins involved in the PI3K/AKT and nuclear factor (NF)-κB signaling pathways. The TUNEL assay was utilized to measure the myocardial apoptosis rate, and immunofluorescence was employed to evaluate the expression of SRSF3 and pathway proteins in a mice model of diabetic cardiomyopathy. Diabetic cardiomyopathy downregulated the expression of SRSF3, which mediated apoptosis through the PI3K/AKT and NF-κB signaling pathways.