[Development of a method for mass production of functional neutrophils derived from human induced pluripotent stem cells].

Toshiya Hino, Mineo Kurokawa
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引用次数: 0

Abstract

Granulocyte transfusion therapy (GTX) is a treatment for severe infections in patients with neutropenia, but is not widely used because it requires repeated apheresis from healthy donors to harvest sufficient neutrophils. Induced pluripotent stem cell (iPSC)-derived neutrophils are considered a promising solution to this problem, and several groups have reported methods for differentiating iPSCs into neutrophils. However, the differentiation process takes more than 14 days, and an expansion culture method that yields sufficient neutrophils for effective GTX must be developed. We have recently established iPSC-derived granulocyte progenitor cell lines that can be expanded in vitro, which could serve as a starting point for supplying sufficient iPSC-derived neutrophils in 4 days when GTX treatment is required. However, before this manufacturing method is ready for clinical use, its efficacy and safety must be evaluated and the method must be improved to comply with quality control standards for regenerative medicine products.

[从人类诱导多能干细胞中大量生产功能性中性粒细胞的方法的发展]。
粒细胞输血疗法(GTX)是一种治疗中性粒细胞减少症患者严重感染的方法,但由于需要从健康供体中反复采珠以获取足够的中性粒细胞,因此并未广泛使用。诱导多能干细胞(iPSC)衍生的中性粒细胞被认为是解决这一问题的一个有希望的解决方案,一些研究小组已经报道了将iPSC分化为中性粒细胞的方法。然而,分化过程需要14天以上,必须开发一种扩增培养方法,以产生足够的中性粒细胞来进行有效的GTX。我们最近建立了ipsc衍生的粒细胞祖细胞系,可以在体外扩增,这可以作为一个起点,当需要GTX治疗时,在4天内提供足够的ipsc衍生中性粒细胞。然而,在这种制造方法用于临床之前,必须对其有效性和安全性进行评估,并对该方法进行改进,以符合再生医学产品的质量控制标准。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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