{"title":"Thermoregulation in goats through AGRP gene and analysis of physiological adaptation.","authors":"Monali Das, Chinmoy Mishra, Smruti Ranjan Mishra, Susanta Kumar Dash, Dillip Kumar Karna, Sukanta Kumar Pradhan, Kumaresh Behera","doi":"10.1007/s10709-025-00240-9","DOIUrl":null,"url":null,"abstract":"<p><p>An association between genetic variation in Agouti Related Peptide (AGRP) gene and adaptability was investigated in Ganjam, Narayanpatana, Maraguda and Keonjhar goat populations of Odisha. The comparative quantitative expression of AGRP gene revealed higher expression in Maraguda and Keonjhar goats as compared to Ganjam and Narayanpatana goats. The bioinformatics analysis revealed the intricate mechanism of action for AGRP protein in the cellular pathway for thermal stress. The three-dimensional structure of AGRP protein was predicted and its stability was ascertained by constructing Ramchandran plot, aromatic receptor surface, hydrogen bond interaction and hydrophobicity plot. Momentous intracellular interaction of AGRP protein with leptin and ghrelin receptors was established through in silico analysis. The relationship between genetic variation with physiological, biochemical and haematological parameters was estimated by calculating the adaptability index (AI) through multivariate analysis. The relative importance of each variable in the adaptive responses of the four goat populations was assessed by path analysis. The difference in AI between the goat populations was slightly over 8%. This preliminary AI provides a potential decision-maker method to scale the resilience level of goats. The path analysis avowed that THI significantly modified the skin temperature and respiration rate which in turn will decrease the rectal temperature showing better adaptability. A comprehensive genetic and phenotypic analysis for thermal resilience in goats was established in this study.</p>","PeriodicalId":55121,"journal":{"name":"Genetica","volume":"153 1","pages":"24"},"PeriodicalIF":1.3000,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetica","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10709-025-00240-9","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
引用次数: 0
Abstract
An association between genetic variation in Agouti Related Peptide (AGRP) gene and adaptability was investigated in Ganjam, Narayanpatana, Maraguda and Keonjhar goat populations of Odisha. The comparative quantitative expression of AGRP gene revealed higher expression in Maraguda and Keonjhar goats as compared to Ganjam and Narayanpatana goats. The bioinformatics analysis revealed the intricate mechanism of action for AGRP protein in the cellular pathway for thermal stress. The three-dimensional structure of AGRP protein was predicted and its stability was ascertained by constructing Ramchandran plot, aromatic receptor surface, hydrogen bond interaction and hydrophobicity plot. Momentous intracellular interaction of AGRP protein with leptin and ghrelin receptors was established through in silico analysis. The relationship between genetic variation with physiological, biochemical and haematological parameters was estimated by calculating the adaptability index (AI) through multivariate analysis. The relative importance of each variable in the adaptive responses of the four goat populations was assessed by path analysis. The difference in AI between the goat populations was slightly over 8%. This preliminary AI provides a potential decision-maker method to scale the resilience level of goats. The path analysis avowed that THI significantly modified the skin temperature and respiration rate which in turn will decrease the rectal temperature showing better adaptability. A comprehensive genetic and phenotypic analysis for thermal resilience in goats was established in this study.
期刊介绍:
Genetica publishes papers dealing with genetics, genomics, and evolution. Our journal covers novel advances in the fields of genomics, conservation genetics, genotype-phenotype interactions, evo-devo, population and quantitative genetics, and biodiversity. Genetica publishes original research articles addressing novel conceptual, experimental, and theoretical issues in these areas, whatever the taxon considered. Biomedical papers and papers on breeding animal and plant genetics are not within the scope of Genetica, unless framed in an evolutionary context. Recent advances in genetics, genomics and evolution are also published in thematic issues and synthesis papers published by experts in the field.