Thermoregulation in goats through AGRP gene and analysis of physiological adaptation.

IF 1.3 4区 生物学 Q4 GENETICS & HEREDITY
Monali Das, Chinmoy Mishra, Smruti Ranjan Mishra, Susanta Kumar Dash, Dillip Kumar Karna, Sukanta Kumar Pradhan, Kumaresh Behera
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引用次数: 0

Abstract

An association between genetic variation in Agouti Related Peptide (AGRP) gene and adaptability was investigated in Ganjam, Narayanpatana, Maraguda and Keonjhar goat populations of Odisha. The comparative quantitative expression of AGRP gene revealed higher expression in Maraguda and Keonjhar goats as compared to Ganjam and Narayanpatana goats. The bioinformatics analysis revealed the intricate mechanism of action for AGRP protein in the cellular pathway for thermal stress. The three-dimensional structure of AGRP protein was predicted and its stability was ascertained by constructing Ramchandran plot, aromatic receptor surface, hydrogen bond interaction and hydrophobicity plot. Momentous intracellular interaction of AGRP protein with leptin and ghrelin receptors was established through in silico analysis. The relationship between genetic variation with physiological, biochemical and haematological parameters was estimated by calculating the adaptability index (AI) through multivariate analysis. The relative importance of each variable in the adaptive responses of the four goat populations was assessed by path analysis. The difference in AI between the goat populations was slightly over 8%. This preliminary AI provides a potential decision-maker method to scale the resilience level of goats. The path analysis avowed that THI significantly modified the skin temperature and respiration rate which in turn will decrease the rectal temperature showing better adaptability. A comprehensive genetic and phenotypic analysis for thermal resilience in goats was established in this study.

AGRP基因对山羊体温调节及生理适应分析。
在印度奥里萨邦的Ganjam、Narayanpatana、Maraguda和Keonjhar山羊群体中研究了Agouti相关肽(AGRP)基因的遗传变异与适应性的关系。AGRP基因在Maraguda和Keonjhar山羊中的表达量高于Ganjam和Narayanpatana山羊。生物信息学分析揭示了AGRP蛋白在热应激细胞通路中的复杂作用机制。通过构建Ramchandran图、芳香受体表面图、氢键相互作用图和疏水性图,预测了AGRP蛋白的三维结构,并确定了其稳定性。通过硅分析证实了AGRP蛋白与瘦素和胃饥饿素受体在细胞内的重要相互作用。通过多变量分析计算适应性指数(AI),估计遗传变异与生理、生化和血液学参数的关系。通过通径分析对四个山羊种群的适应反应中各变量的相对重要性进行了评估。山羊种群之间的人工智能差异略高于8%。这种初步的人工智能为决策者提供了一种衡量山羊恢复能力水平的潜在方法。通径分析表明THI显著改变皮肤温度和呼吸速率,进而降低直肠温度,表现出更好的适应性。本研究对山羊的热恢复能力进行了全面的遗传和表型分析。
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来源期刊
Genetica
Genetica 生物-遗传学
CiteScore
2.70
自引率
0.00%
发文量
32
审稿时长
>12 weeks
期刊介绍: Genetica publishes papers dealing with genetics, genomics, and evolution. Our journal covers novel advances in the fields of genomics, conservation genetics, genotype-phenotype interactions, evo-devo, population and quantitative genetics, and biodiversity. Genetica publishes original research articles addressing novel conceptual, experimental, and theoretical issues in these areas, whatever the taxon considered. Biomedical papers and papers on breeding animal and plant genetics are not within the scope of Genetica, unless framed in an evolutionary context. Recent advances in genetics, genomics and evolution are also published in thematic issues and synthesis papers published by experts in the field.
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