The Yarkand hare epidermal growth factor receptor improves the survival and antioxidant capacity of HeLa cells under stress.

IF 2 2区 生物学 Q2 ZOOLOGY
Current Zoology Pub Date : 2025-05-02 eCollection Date: 2025-06-01 DOI:10.1093/cz/zoaf020
Yuge Cui, Mengqi Xu, Mingchang Duan, Jinshan Wu, Yanyu Zhou, Wenjuan Shan
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Abstract

Epidermal growth factor receptor (EGFR) is one of the most important tyrosine kinase receptor families, which plays a pivotal role in cell signaling transduction and physiological processes. Studies on the EGFR gene in humans and other species have demonstrated its pivotal role in regulating the sodium ion balance and mediating sodium and water reabsorption in the kidney's proximal tubules. However, the impact of EGFR gene in how the Yarkand hare (Lepus yarkandensis) adapts to extreme environmental habitat remains unclear. The Yarkand hare is a desert-dwelling animal with multiple adaptations to cope with drought. Given the important physiological function of EGFR gene, we strived to understand its role in arid environment and explore the molecular mechanism of drought tolerance in the Yarkand hare. We first performed segmental cloning of the CDS of the Yarkand hare EGFR gene. Then, we constructed the phylogenetic tree of the Yarkand hare's EGFR gene and compared it with that of other species. The results showed that the Yarkand hare was most closely related to the Tolai hare (Lepus tolai). Through quantitative reverse transcription polymerase chain reaction (RT-qPCR), we discovered that EGFR expression in the kidneys of the Yarkand hare was higher than in the allopatric Tolai hare from non-arid areas. Therefore, we hypothesized that EGFR gene overexpression in the kidney of the Yarkand hare may play a crucial role in drought adaptability. Subsequently, we inserted CDS of EGFR gene into a pcDNA3.1-EGFP expression vector to construct recombinant plasmid, which was transfected into HeLa cells and overexpressed. RT-qPCR demonstrated a notable and statistically significant increase in EGFR mRNA expression and western blot proved stable expression of this protein in HeLa cells. Through cell experiments, EGFR gene overexpression markedly enhanced the survival of Hela cells subjected to NaCl, H2O2, and heat stresses, increased superoxide dismutase activity, and decreased malondialdehyde content. In conclusion, these findings preliminarily suggest that EGFR might help the Yarkand hare adapt to extreme environmental conditions. EGFR manipulation in vivo could be a promising strategy to enhance the resilience of animals to extreme conditions.

Yarkand hare表皮生长因子受体可提高HeLa细胞在应激条件下的存活率和抗氧化能力。
表皮生长因子受体(Epidermal growth factor receptor, EGFR)是酪氨酸激酶最重要的受体家族之一,在细胞信号转导和生理过程中起着关键作用。对人类和其他物种EGFR基因的研究表明,它在调节肾近端小管钠离子平衡和介导钠和水的重吸收中起着关键作用。然而,EGFR基因在小野兔(Lepus yarkandensis)如何适应极端环境栖息地中的影响尚不清楚。yardand野兔是一种生活在沙漠中的动物,具有多种适应干旱的能力。鉴于EGFR基因具有重要的生理功能,我们努力了解其在干旱环境中的作用,探索旱地兔抗旱的分子机制。我们首先对Yarkand hare EGFR基因的CDS进行了片段克隆。在此基础上,构建了叶家兔EGFR基因的系统发育树,并与其他物种进行了比较。结果表明,Yarkand hare与Tolai hare (Lepus Tolai)亲缘关系最近。通过定量逆转录聚合酶链反应(RT-qPCR),我们发现Yarkand野兔肾脏中EGFR的表达高于来自非干旱地区的Tolai野兔。因此,我们假设EGFR基因在家兔肾脏中的过表达可能在干旱适应性中起关键作用。随后,我们将EGFR基因CDS插入pcDNA3.1-EGFP表达载体中构建重组质粒,转染HeLa细胞并进行过表达。RT-qPCR结果显示EGFR mRNA表达显著升高,且有统计学意义;western blot结果显示该蛋白在HeLa细胞中稳定表达。通过细胞实验,EGFR基因过表达可显著提高NaCl、H2O2和热应激下Hela细胞的存活率,提高超氧化物歧化酶活性,降低丙二醛含量。综上所述,这些结果初步提示EGFR可能有助于Yarkand适应极端环境条件。在体内操纵EGFR可能是一种很有前途的策略,可以增强动物对极端条件的适应能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Current Zoology
Current Zoology Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
3.20
自引率
9.10%
发文量
111
审稿时长
6 weeks
期刊介绍: About the Journal Current Zoology (formerly Acta Zoologica Sinica, founded in 1935) is an open access, bimonthly, peer-reviewed international journal of zoology. It publishes review articles and research papers in the fields of ecology, evolution and behaviour. Current Zoology is sponsored by Institute of Zoology, Chinese Academy of Sciences, along with the China Zoological Society.
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