Maternal thyroid hormones affect zebrafish embryo development

IF 3.6 3区医学 Q2 CELL BIOLOGY

Molecular and Cellular Endocrinology Pub Date : 2025-07-05 DOI:10.1016/j.mce.2025.112610

Maira da Silva Rodrigues , Beatriz Marques de Souza , Ivana Felipe Rosa , Lucas Benites Doretto , Rafael Henrique Nóbrega

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Abstract

Thyroid hormones (THs), including thyroxine (T4) and triiodothyronine (T3), regulate vertebrate development, influencing critical processes during embryogenesis and larval stages. However, their maternal transfer and developmental roles in fish remain poorly understood. This study investigates maternal THs' impact on zebrafish development, focusing on the somatotropic axis, a key regulator of growth and metabolism. Female zebrafish were fed diets with 12.5 or 25 μg T4/g body weight/day for five days, while controls received vehicle-only food. Plasma TH levels were measured, and females were bred with untreated males. Embryos and larvae were assessed from 0 to 144 h post-fertilization for survival, heartbeat, morphology, T3 content, and gene expression. Elevated maternal T4 increased embryonic T3, causing growth retardation, edema, spinal deformities, tachycardia, and mortality. Gene expression analysis revealed dysregulation of TH axis components and somatotropic genes, highlighting the maternal THs' critical role in developmental trajectories and normal growth.

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母体甲状腺激素影响斑马鱼胚胎发育。

甲状腺激素（THs），包括甲状腺素（T4）和三碘甲状腺原氨酸（T3），调节脊椎动物的发育，影响胚胎发生和幼虫阶段的关键过程。然而，它们在鱼类中的母体转移和发育作用仍然知之甚少。本研究探讨了母体THs对斑马鱼发育的影响，重点关注了生长和代谢的关键调节因子——体致轴。雌性斑马鱼饲喂12.5或25 μg T4/g体重/天的饲料，连续5天，对照组饲喂纯饲料。测量血浆TH水平，并将雌性与未经治疗的雄性交配。在受精后0 ~ 144小时评估胚胎和幼虫的存活、心跳、形态、T3含量和基因表达。母体T4升高会增加胚胎T3，导致生长迟缓、水肿、脊柱畸形、心动过速和死亡。基因表达分析揭示了TH轴组分和促生长基因的失调，突出了母体TH在发育轨迹和正常生长中的关键作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular and Cellular Endocrinology 医学-内分泌学与代谢

CiteScore

9.00

自引率

2.40%

发文量

174

审稿时长

42 days

期刊介绍： Molecular and Cellular Endocrinology was established in 1974 to meet the demand for integrated publication on all aspects related to the genetic and biochemical effects, synthesis and secretions of extracellular signals (hormones, neurotransmitters, etc.) and to the understanding of cellular regulatory mechanisms involved in hormonal control.