Identification of candidate CircRNA associated with intramuscular fat deposition in Duroc pigs

IF 3.4 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2025-07-04 DOI:10.1016/j.ygeno.2025.111073

Yue Chen , Xiaohong Hou , Yawei Fu , Hu Gao , Qian Wang , Ningyu Fan , Qizheng Xie , Ruimin Ren , Fengming Chen , Yulong Yin , Kang Xu

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Abstract

In order to investigate the potential mechanisms affecting the intramuscular fat content, high-throughput RNA sequencing was employed to assess the expression profiles of circRNAs in the longissimus dorsi tissues of Duroc pigs with high and low IMF content. A total of 230 circRNAs showed significantly differential expression levels between the HIMF and LIMF groups. GO annotation revealed that the host genes of these circRNAs were associated with responses to lipids, such as lipid tube assembly, lipid metabolic process, etc. Moreover, the KEGG enrichment analysis indicated that the host genes of these circRNAs were enriched in the AMP-activated protein kinase signaling pathway, fatty acid metabolism, etc. Several key circRNAs involved in lipid metabolism were screened, including novel_circ_0001751, novel_circ_0018596, novel_circ_0000494, etc. In addition, five circRNA-miRNA interaction networks were constructed. Notably, this study demonstrates for the first time the role of novel_circ_0018596 and novel_circ_0000494 in controlling IMF deposition in Duroc pigs.

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杜洛克猪肌内脂肪沉积相关候选环状rna的鉴定

为了研究影响肌内脂肪含量的潜在机制，采用高通量RNA测序方法评估了IMF含量高和低的杜洛克猪背最长肌组织中环状RNA的表达谱。在HIMF组和LIMF组中，共有230个circrna的表达水平存在显著差异。GO注释显示，这些circRNAs的宿主基因与脂质反应有关，如脂质管组装、脂质代谢过程等。此外，KEGG富集分析表明，这些环状rna的宿主基因富集于amp激活的蛋白激酶信号通路、脂肪酸代谢等。筛选了几个参与脂质代谢的关键circrna，包括novel_circ_0001751、novel_circ_0018596、novel_circ_0000494等。此外，构建了5个circRNA-miRNA相互作用网络。值得注意的是，本研究首次证明了novel_circ_0018596和novel_circ_0000494在控制杜洛克猪体内IMF沉积中的作用。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.