Potential of biochar-based inoculant in enhancing rhizobia survival and grain yield of cowpea (Vigna unguiculata (L.) Walp.)

IF 1.5 Q3 AGRONOMY
Winnefred Mensah, Nana Ewusi-Mensah, Jacob Ulzen, Ophelia Osei Ulzen, Azumah Ayamah
{"title":"Potential of biochar-based inoculant in enhancing rhizobia survival and grain yield of cowpea (Vigna unguiculata (L.) Walp.)","authors":"Winnefred Mensah,&nbsp;Nana Ewusi-Mensah,&nbsp;Jacob Ulzen,&nbsp;Ophelia Osei Ulzen,&nbsp;Azumah Ayamah","doi":"10.1002/agg2.70161","DOIUrl":null,"url":null,"abstract":"<p>The need for low-cost carrier materials is paramount for rhizobium inoculants production, especially in sub-Saharan Africa. This research studied different feedstocks biochar as potential carriers for inoculant production. Coconut coir (CC), coconut shell (CS), rice husk (RH), and sugarcane bagasse (SB) biochars were used as carriers, and their performance was compared to that of standard industrial peat (control). The biochar and peat carriers were inoculated with two elite <i>Bradyrhizobium</i> strains such as KNUST 1002 and KNUST 1006 and the reference strain BR 3267 (where BR is <i>Bradyrhizobium</i>). The survival rate of the rhizobia was monitored by determining the number of cells, pH, and moisture content for 24 weeks. In addition, the effectiveness of different inoculated carriers was tested in cowpea (<i>Vigna unguiculata</i> (L.) Walp) under field conditions via a randomized complete block design with four replications. CC and SB enhanced <i>Bradyrhizobium</i> survival for up to 16 and 20 weeks, respectively, whereas CS and RH promoted strain survival for up to 8 weeks. The viable cell count was highest in the peat-based inoculant during the 24-week period. BR 3267 in CC and SB significantly (<i>p</i> &lt; 0.001) increased the nodule dry weight of cowpea compared to uninoculated control. The highest grain yield (1774 kg ha<sup>−1</sup>) was recorded for CC, which was 16% greater than the grain yield of the peat-based inoculant (1524 kg ha<sup>−1</sup>), although this difference was not significant. CC biochar is a potential carrier for inoculant production in Ghana.</p>","PeriodicalId":7567,"journal":{"name":"Agrosystems, Geosciences & Environment","volume":"8 3","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/agg2.70161","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agrosystems, Geosciences & Environment","FirstCategoryId":"1085","ListUrlMain":"https://acsess.onlinelibrary.wiley.com/doi/10.1002/agg2.70161","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0

Abstract

The need for low-cost carrier materials is paramount for rhizobium inoculants production, especially in sub-Saharan Africa. This research studied different feedstocks biochar as potential carriers for inoculant production. Coconut coir (CC), coconut shell (CS), rice husk (RH), and sugarcane bagasse (SB) biochars were used as carriers, and their performance was compared to that of standard industrial peat (control). The biochar and peat carriers were inoculated with two elite Bradyrhizobium strains such as KNUST 1002 and KNUST 1006 and the reference strain BR 3267 (where BR is Bradyrhizobium). The survival rate of the rhizobia was monitored by determining the number of cells, pH, and moisture content for 24 weeks. In addition, the effectiveness of different inoculated carriers was tested in cowpea (Vigna unguiculata (L.) Walp) under field conditions via a randomized complete block design with four replications. CC and SB enhanced Bradyrhizobium survival for up to 16 and 20 weeks, respectively, whereas CS and RH promoted strain survival for up to 8 weeks. The viable cell count was highest in the peat-based inoculant during the 24-week period. BR 3267 in CC and SB significantly (p < 0.001) increased the nodule dry weight of cowpea compared to uninoculated control. The highest grain yield (1774 kg ha−1) was recorded for CC, which was 16% greater than the grain yield of the peat-based inoculant (1524 kg ha−1), although this difference was not significant. CC biochar is a potential carrier for inoculant production in Ghana.

Abstract Image

Abstract Image

Abstract Image

生物炭基接种剂提高豇豆根瘤菌存活率和产量的潜力Walp)。
对低成本载体材料的需求对根瘤菌接种剂的生产至关重要,特别是在撒哈拉以南非洲。本研究研究了不同原料生物炭作为孕育剂生产的潜在载体。以椰壳(CC)、椰壳(CS)、稻壳(RH)和甘蔗渣(SB)生物炭为载体,与标准工业泥炭(对照)进行了性能比较。分别用KNUST 1002和KNUST 1006两株慢生根瘤菌和参考菌株br3267 (BR为慢生根瘤菌)接种生物炭和泥炭载体。通过测定细胞数、pH值和水分含量来监测根瘤菌的存活率,持续24周。此外,还对不同载体在豇豆(Vigna unguiculata, L.)中的接种效果进行了试验。Walp)在野外条件下通过随机完全区组设计,共4个重复。CC和SB分别可提高缓生根瘤菌的存活时间达16周和20周,而CS和RH可提高菌株的存活时间达8周。在24周内,泥炭基接种剂的活细胞数最高。BR 3267在CC和SB中的表达显著(p <;0.001),与未接种的对照相比,豇豆根瘤干重增加。CC的籽粒产量最高(1774 kg ha - 1),比泥炭基接种剂的籽粒产量(1524 kg ha - 1)高16%,但差异不显著。CC生物炭是加纳生产接种剂的潜在载体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Agrosystems, Geosciences & Environment
Agrosystems, Geosciences & Environment Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
2.60
自引率
0.00%
发文量
80
审稿时长
24 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信