A practical look at cryo-electron tomography image processing: Key considerations for new biological discoveries

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current opinion in structural biology Pub Date : 2025-07-08 DOI:10.1016/j.sbi.2025.103116

William Wan

引用次数: 0

Abstract

Cryo-electron tomography (cryo-ET) enables 3D visualization of complex biological environments without the need for purification, thereby preserving the native biological context of the specimen. For determining macromolecular structures, repeating molecules can be localized in tomograms and subjected to subtomogram averaging, the 3D analog to single particle analysis. In addition to molecular structure, tomograms have a wealth of other information that can be accessed through image processing, including the analysis of membrane surfaces, cytoskeletal filaments, and the relationships between molecules of interest. Here, we provide an overview of recent developments in cryo-ET image processing with the goal of clarifying key considerations to help new users obtain novel biological findings.

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冷冻电子断层成像图像处理的实际观察：新生物学发现的关键考虑因素

低温电子断层扫描（cryo-ET）能够在不需要纯化的情况下实现复杂生物环境的3D可视化，从而保留标本的原生生物环境。为了确定大分子结构，重复分子可以在层析图中定位并进行亚层析图平均，这是单粒子分析的3D模拟。除了分子结构外，断层摄影还可以通过图像处理获得丰富的其他信息，包括膜表面分析，细胞骨架细丝，以及感兴趣的分子之间的关系。在这里，我们概述了冷冻et图像处理的最新发展，目的是澄清关键考虑因素，以帮助新用户获得新的生物学发现。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation