Lipid asymmetry and membrane trafficking: transbilayer distribution of structural phospholipids as regulators of exocytosis and endocytosis.

IF 4 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Biological Chemistry Pub Date : 2025-07-01 DOI:10.1016/j.jbc.2025.110441

Margherita Caputo, Olga Gubar, Petra Tóth, Nicolas Vitale, Stéphane Gasman, Stéphane Ory

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Abstract

The plasma membrane of eukaryotic cells is highly dynamic and asymmetrically organized. Its continuous remodeling plays a crucial role in diverse cellular processes, including apoptosis, blood coagulation, and vesicular trafficking. The distribution and rearrangement of phospholipids (PLs) within the bilayer are tightly regulated, influencing membrane curvature, tension, and organization. This review examines the role of PL asymmetry in vesicle fusion, the final step of exocytosis, and in vesicular membrane retrieval by compensatory endocytosis in neurosecretory cells, with a particular emphasis on structural PLs such as phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylserine (PS). We discuss the molecular mechanisms that maintain and disrupt PLs asymmetry and explore how lipid rearrangements affect vesicle dynamics. Additionally, we highlight recent findings on lipid scramblases, particularly phospholipid scramblase-1 (PLSCR1), and their role in regulated exocytosis and compensatory endocytosis.

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脂质不对称和膜运输：结构磷脂作为胞吐和内吞调节因子的跨双层分布。

真核细胞的质膜是高度动态和不对称组织的。它的持续重塑在多种细胞过程中起着至关重要的作用，包括细胞凋亡、血液凝固和囊泡运输。磷脂（PLs）在双分子层内的分布和重排受到严格调控，影响膜的曲率、张力和组织。这篇综述探讨了聚乳酸不对称在囊泡融合中的作用，囊泡融合是胞外分泌的最后一步，在神经分泌细胞代偿性内吞作用下的囊泡膜恢复中，特别强调了结构性聚乳酸，如磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）和磷脂酰丝氨酸（PS）。我们讨论了维持和破坏PLs不对称的分子机制，并探讨了脂质重排如何影响囊泡动力学。此外，我们强调了最近关于脂质超燃酶的发现，特别是磷脂超燃酶-1 (PLSCR1)，以及它们在调节胞吐和代偿性内吞中的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biological Chemistry Biochemistry, Genetics and Molecular Biology-Biochemistry

自引率

4.20%

发文量

1233

期刊介绍： The Journal of Biological Chemistry welcomes high-quality science that seeks to elucidate the molecular and cellular basis of biological processes. Papers published in JBC can therefore fall under the umbrellas of not only biological chemistry, chemical biology, or biochemistry, but also allied disciplines such as biophysics, systems biology, RNA biology, immunology, microbiology, neurobiology, epigenetics, computational biology, ’omics, and many more. The outcome of our focus on papers that contribute novel and important mechanistic insights, rather than on a particular topic area, is that JBC is truly a melting pot for scientists across disciplines. In addition, JBC welcomes papers that describe methods that will help scientists push their biochemical inquiries forward and resources that will be of use to the research community.