Comparative analysis of 3D culture methodologies in prostate cancer cells

IF 3.5 3区 生物学 Q3 CELL BIOLOGY
Ella Foster , Oliver Wardhana , Ziyu Zeng , Xin Lu
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引用次数: 0

Abstract

Three-dimensional (3D) cell culture models are increasingly utilized in cancer research to better replicate in vivo tumor microenvironments. This study examines the effects of different 3D scaffolding materials, including Matrigel, GelTrex, and the plant-based GrowDex, on prostate cancer cell lines, with a particular emphasis on neuroendocrine prostate cancer (NEPC). Four cell lines (LNCaP, LASCPC-01, PC-3, and KUCaP13) were cultured in these scaffolds using the sandwich method to evaluate spheroid formation, cell viability, and gene expression. The results revealed that while all scaffolds supported cell viability, spheroid formation varied significantly: Matrigel promoted the most consistent spheroid formation, exhibiting the best results for LASCPC-01 among the tested scaffolds, though spheroid formation for LASCPC-01 was generally more limited than for LNCaP. Gene expression analysis indicated a reduction in androgen receptor (AR) expression in LNCaP cells across all scaffolds using the sandwich method. However, further experiments using a mini-dome method revealed that the expression of AR signaling genes and neuroendocrine marker genes varied depending on the scaffolds and culture methods. These findings highlight the scaffold-dependent variability in 3D culture outcomes and emphasize the need for standardized methodologies to ensure consistency and relevance in prostate cancer research.
前列腺癌细胞三维培养方法的比较分析。
三维(3D)细胞培养模型越来越多地用于癌症研究,以更好地复制体内肿瘤微环境。本研究考察了不同3D支架材料(包括Matrigel、GelTrex和植物基GrowDex)对前列腺癌细胞系的影响,特别强调了神经内分泌前列腺癌(NEPC)。采用夹心法在这些支架中培养4株细胞系(LNCaP、LASCPC-01、PC-3和KUCaP13),评估球体形成、细胞活力和基因表达。结果显示,虽然所有支架都支持细胞活力,但球体形成的差异很大:Matrigel促进的球体形成最一致,在测试的支架中LASCPC-01表现出最好的效果,尽管LASCPC-01的球体形成通常比LNCaP更有限。基因表达分析表明,在所有支架中使用三明治方法的LNCaP细胞雄激素受体(AR)表达降低。然而,使用迷你穹顶法的进一步实验表明,AR信号基因和神经内分泌标记基因的表达因支架和培养方法而异。这些发现强调了3D培养结果中支架依赖性的可变性,并强调了标准化方法的必要性,以确保前列腺癌研究的一致性和相关性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Experimental cell research
Experimental cell research 医学-细胞生物学
CiteScore
7.20
自引率
0.00%
发文量
295
审稿时长
30 days
期刊介绍: Our scope includes but is not limited to areas such as: Chromosome biology; Chromatin and epigenetics; DNA repair; Gene regulation; Nuclear import-export; RNA processing; Non-coding RNAs; Organelle biology; The cytoskeleton; Intracellular trafficking; Cell-cell and cell-matrix interactions; Cell motility and migration; Cell proliferation; Cellular differentiation; Signal transduction; Programmed cell death.
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