Antioxidant and Anti-Fatigue Properties of Cucumaria frondosa Abdominal Hydrolysates in an Endurance Swimming Mouse Model

IF 3.2 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY
Jiarun Gao, Shunmin Gong, Shijia Huang, Mingbo Li, Leilei Sun
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Abstract

Cucumaria frondosa abdominal hydrolysates (CFHs) were prepared using flavorzyme under optimal conditions, and their antioxidant activity, stability, and anti-fatigue activity were systematically evaluated. CFHs exhibited excellent free radical scavenging ability at temperatures below 60°C and under neutral to weakly acidic conditions. Under optimal conditions, the hydroxyl radical scavenging activity of CFHs reached as high as 81.48%, with reducing activity up to 76.35% and Fe2+-chelating activity of 46.36%. In our study on anti-fatigue in mice, we utilized male ICR mice as experimental subjects and ginseng as the positive control. Forced swimming model experiments were conducted to assess the anti-fatigue effects of CFHs, which were measured through swimming duration, hematoxylin and eosin (H&E) staining of liver tissues, and various assay kits. Compared to the control group, CFHs significantly prolonged the forced swimming time to 12.79 min, outperforming ginseng. In addition, CFHs improved the glycogen reserve and antioxidant system, as well as reduced the expression levels of lactic acid (LA), ammonia (NH3), and blood urea nitrogen (BUN). Liver glycogen and blood glucose contents were significantly elevated in all three groups of mice treated with CFHs via gavage for 30 days. Concurrently, CFHs upregulated superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), alleviating muscle damage. These findings suggest that CFHs may alleviate fatigue through multiple targeted mechanisms, including energy optimization, oxidative stress reduction, and inhibition of metabolic waste.

灰树瓜腹部水解液对耐力游泳小鼠模型的抗氧化和抗疲劳性能
在最佳工艺条件下,利用风味酶制备了灰树瓜腹水解液(CFHs),并对其抗氧化、稳定性和抗疲劳活性进行了系统评价。CFHs在60℃以下和中性至弱酸性条件下均表现出良好的自由基清除能力。在最优条件下,CFHs对羟基自由基的清除活性高达81.48%,还原活性高达76.35%,Fe2+螯合活性为46.36%。在小鼠抗疲劳研究中,我们以雄性ICR小鼠为实验对象,以人参为阳性对照。采用强迫游泳模型实验,通过游泳时间、肝组织苏木精和伊红(H&;E)染色及各种检测试剂盒检测CFHs的抗疲劳作用。与对照组相比,CFHs显著延长了强迫游泳时间,达到12.79 min,优于人参。此外,CFHs改善了糖原储备和抗氧化系统,降低了乳酸(LA)、氨(NH3)和血尿素氮(BUN)的表达水平。三组小鼠灌胃CFHs 30 d后,肝糖原和血糖含量均显著升高。同时,CFHs上调超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px),减轻肌肉损伤。这些发现表明,CFHs可能通过多种靶向机制缓解疲劳,包括能量优化、氧化应激减少和代谢废物抑制。
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来源期刊
Journal of Food Science
Journal of Food Science 工程技术-食品科技
CiteScore
7.10
自引率
2.60%
发文量
412
审稿时长
3.1 months
期刊介绍: The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science. The range of topics covered in the journal include: -Concise Reviews and Hypotheses in Food Science -New Horizons in Food Research -Integrated Food Science -Food Chemistry -Food Engineering, Materials Science, and Nanotechnology -Food Microbiology and Safety -Sensory and Consumer Sciences -Health, Nutrition, and Food -Toxicology and Chemical Food Safety The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.
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