Minocycline reduces proinflammatory and oxidative stress markers in the spinal cord and morphology changes in sciatic nerve of Type 2 diabetic neuropathy rat model.

IF 1.2 Q4 ENDOCRINOLOGY & METABOLISM
Diabetology International Pub Date : 2025-03-19 eCollection Date: 2025-07-01 DOI:10.1007/s13340-025-00811-3
Norhamidar Ab Hamid, Norsuhana Omar, Che Aishah Nazariah Ismail, Idris Long
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Abstract

Aim: This study investigated the effects of minocycline on proinflammatory cytokines, oxidative stress marker levels in the spinal cord and sciatic nerve morphology in Type 2 diabetic (T2DM) neuropathy rats.

Methods: Male Sprague Dawley rats were randomly assigned to six groups (n = 14 per groups): Control (C), T2DM control (STZ), T2DM treated with minocycline 40 mg/kg (STZ + M40) and 80 mg/kg (STZ + M80), T2DM treated with gabapentin (STZ + G10) and non-painful T2DM neuropathy (NPDN). T2DM was induced in obese rats using a combination of high fat diet (HFD) and low-dose streptozotocin (STZ) (40 mg/kg) injection. Then, the neuropathic pain behaviour, body weight and blood biochemical analysis were performed. Rats were sacrificed and the spinal cord and sciatic nerve were collected for ELISA and histology examination.

Results: T2DM rat groups were significantly increased body weight after 6 weeks but significantly reduced from 8 until 9 weeks compared to control group (p < 0.05). The fasting blood glucose (FBG) level in all T2DM groups were significantly higher on day 3, day 14, and day 22 compared to control group (p < 0.05) consistent with HbA1c levels. T2DM groups also significantly increased MDA, TNF-α, IL-1β and C-Reactive Protein (CRP) but decreased SOD and Catalase levels in the spinal cord compared to control group (p < 0.05). T2DM groups also showed significant abnormal morphology changes in the sciatic nerve compared to control group (p < 0.05). Minocycline dependent on doses and gabapentin in T2DM rat significantly alleviated all these effects.

Conclusion: These findings suggest the neuroprotective effects of minocycline on T2DM neuropathy.

米诺环素降低2型糖尿病神经病变大鼠脊髓促炎和氧化应激标志物及坐骨神经形态学改变。
目的:研究米诺环素对2型糖尿病(T2DM)神经病变大鼠脊髓促炎细胞因子、氧化应激标志物水平及坐骨神经形态学的影响。方法:雄性Sprague Dawley大鼠随机分为6组(每组n = 14):对照组(C)、T2DM对照组(STZ)、米诺环素40 mg/kg (STZ + M40)和80 mg/kg (STZ + M80)、加巴喷丁(STZ + G10)和非疼痛性T2DM神经病(NPDN)。采用高脂饮食(HFD)联合注射低剂量链脲佐菌素(STZ) (40 mg/kg)诱导肥胖大鼠T2DM。然后进行神经性疼痛行为、体重和血液生化分析。处死大鼠,采集脊髓和坐骨神经进行酶联免疫吸附试验和组织学检查。结果:T2DM组大鼠体重在6周后显著增加,而在8 ~ 9周显著降低(p p p p)。结论:提示二甲胺四环素对T2DM神经病变具有神经保护作用。
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来源期刊
Diabetology International
Diabetology International ENDOCRINOLOGY & METABOLISM-
CiteScore
3.90
自引率
4.50%
发文量
42
期刊介绍: Diabetology International, the official journal of the Japan Diabetes Society, publishes original research articles about experimental research and clinical studies in diabetes and related areas. The journal also presents editorials, reviews, commentaries, reports of expert committees, and case reports on any aspect of diabetes. Diabetology International welcomes submissions from researchers, clinicians, and health professionals throughout the world who are interested in research, treatment, and care of patients with diabetes. All manuscripts are peer-reviewed to assure that high-quality information in the field of diabetes is made available to readers. Manuscripts are reviewed with due respect for the author''s confidentiality. At the same time, reviewers also have rights to confidentiality, which are respected by the editors. The journal follows a single-blind review procedure, where the reviewers are aware of the names and affiliations of the authors, but the reviewer reports provided to authors are anonymous. Single-blind peer review is the traditional model of peer review that many reviewers are comfortable with, and it facilitates a dispassionate critique of a manuscript.
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