Engineered Yeasts Displaying PETase and MHETase as Whole-Cell Biocatalysts for the Degradation of Polyethylene Terephthalate (PET).

IF 3.7 2区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

ACS Synthetic Biology Pub Date : 2025-07-18 Epub Date: 2025-07-02 DOI:10.1021/acssynbio.5c00209

Caiping Jiang, Kairui Zhai, R Clay Wright, Juhong Chen

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引用次数: 0

Abstract

Due to its low cost of manufacturing, poly(ethylene terephthalate) (PET, a polyester plastic) has been the most widely used plastic material for food packaging. However, PET is nonbiodegradable. It can take years to degrade when it is discarded into the environment. In recent years, plastic pollution has received much attention and has become a major environmental issue. In this study, we engineered yeast surfaces to display two PET-degrading enzymes (PETase and MHETase) to degrade PET plastics. The enzymes displayed on the yeast surface were characterized by using confocal microscopy and flow cytometry. The reaction conditions to degrade PET plastics using the engineered yeasts were optimal at pH 9 and 30 °C. In addition, the engineered yeasts showed great stability and reusability to degrade PET films. Furthermore, we demonstrated that the engineered yeasts as whole-cell catalysts can be used to degrade drinking water bottles into value-added products. This study provides a novel whole-cell biocatalyst using engineered yeasts to degrade plastic waste, offering a new strategy to solve plastic pollution and recycling challenges.

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展示PETase和MHETase作为降解聚对苯二甲酸乙二醇酯（PET）全细胞生物催化剂的工程酵母。

由于制造成本低，聚对苯二甲酸乙酯（PET，一种聚酯塑料）一直是食品包装中应用最广泛的塑料材料。然而，PET是不可生物降解的。当它被丢弃到环境中时，可能需要数年才能降解。近年来，塑料污染受到了广泛关注，并已成为一个重大的环境问题。在这项研究中，我们设计酵母表面显示两种PET降解酶（PETase和MHETase）来降解PET塑料。利用共聚焦显微镜和流式细胞术对酵母表面的酶进行了表征。用工程酵母降解PET塑料的最佳反应条件为pH 9和30℃。此外，工程酵母在降解PET薄膜方面表现出良好的稳定性和可重复使用性。此外，我们还证明了工程酵母作为全细胞催化剂可用于将饮用水瓶降解为增值产品。本研究提供了一种利用工程酵母降解塑料垃圾的新型全细胞生物催化剂，为解决塑料污染和回收挑战提供了一种新的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Synthetic Biology 生物-

CiteScore

8.00

自引率

10.60%

发文量

380

审稿时长

6-12 weeks

期刊介绍： The journal is particularly interested in studies on the design and synthesis of new genetic circuits and gene products; computational methods in the design of systems; and integrative applied approaches to understanding disease and metabolism. Topics may include, but are not limited to: Design and optimization of genetic systems Genetic circuit design and their principles for their organization into programs Computational methods to aid the design of genetic systems Experimental methods to quantify genetic parts, circuits, and metabolic fluxes Genetic parts libraries: their creation, analysis, and ontological representation Protein engineering including computational design Metabolic engineering and cellular manufacturing, including biomass conversion Natural product access, engineering, and production Creative and innovative applications of cellular programming Medical applications, tissue engineering, and the programming of therapeutic cells Minimal cell design and construction Genomics and genome replacement strategies Viral engineering Automated and robotic assembly platforms for synthetic biology DNA synthesis methodologies Metagenomics and synthetic metagenomic analysis Bioinformatics applied to gene discovery, chemoinformatics, and pathway construction Gene optimization Methods for genome-scale measurements of transcription and metabolomics Systems biology and methods to integrate multiple data sources in vitro and cell-free synthetic biology and molecular programming Nucleic acid engineering.