Human papillomavirus 16 mitigates Sneathia vaginalis-induced damage to cervical keratinocytes.

IF 3.7 2区 生物学 Q2 MICROBIOLOGY
mSphere Pub Date : 2025-07-01 DOI:10.1128/msphere.00152-25
Phoebe V Bridy, Jasmine C Cruz, Jada L Covington, Taharah I Islam, Catherine E Hadley, Kayla Tran, Rachel Fry, Bradley A Sheffield, Myrna Serrano, Gregory A Buck, Jinlei Zhao, Katherine Y Tossas, Craig Meyers, Iain M Morgan, Claire D James, Kimberly K Jefferson
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Abstract

Sneathia vaginalis is a bacterial component of the vaginal microbiome that is of clinical interest because of its association with preterm birth and other obstetric complications. It produces a cytotoxin, but little is known about the mechanism through which it kills epithelial cells or the role that cytotoxicity plays in bacterial survival. Recent microbiome studies demonstrate an association between S. vaginalis and human papillomavirus (HPV) within the female reproductive tract, suggesting that HPV and S. vaginalis could interact in some way within this shared niche. We analyzed 16S rRNA survey and HPV typing data from our Vaginal Human Microbiome Project and found, in agreement with other reports, that S. vaginalis was associated with HPV infection. To test the hypothesis that HPV promotes the growth of S. vaginalis, growth and cytotoxicity of S. vaginalis in co-culture with HPV16-positive and HPV-negative human cervical keratinocytes (HCK) were quantitatively assessed. Organotypic HCK rafts expressing HPV16 were more resistant to S. vaginalis-induced damage, as assessed by histology, and supported increased bacterial growth relative to HPV-negative HCK rafts. When S. vaginalis was co-cultured with HPV16-positive and HPV-negative HCK monolayers, cytotoxicity was observed in both HPV16-positive and HPV-negative cells, but HPV16-positive cells were more resistant to the toxic effects of the bacteria and supported bacterial growth for an extended period of time. In conclusion, HPV16 may protect cervical keratinocytes from the cytotoxic effects of S. vaginalis, preventing the eradication of colonized cells and supporting bacterial growth, and this could underlie the association between S. vaginalis and HPV in vivo.IMPORTANCESneathia vaginalis (S. vaginalis) is a bacterial species that lives in the human vagina and can cause complications during pregnancy if it invades the uterus. It is capable of killing cervical epithelial cells. Human papillomaviruses (HPV) are sexually transmitted viruses that can cause genital lesions and cervical cancer. Recently, multiple reports describe an association between S. vaginalis and HPV. This study used cultured cervical epithelial cells expressing the high-risk HPV type, HPV16, and HPV-negative cells to determine whether HPV promotes the growth of S. vaginalis. We found that HPV16 promotes the survival of cervical epithelial cells that are exposed to S. vaginalis. Survival of cervical epithelial cells may benefit the growth of S. vaginalis, which adhere to and feed off of these cells to survive in the female reproductive tract.

人乳头瘤病毒16减轻阴道炎引起的宫颈角化细胞损伤。
阴道Sneathia是阴道微生物组的一种细菌组成部分,由于其与早产和其他产科并发症有关,因此具有临床意义。它产生一种细胞毒素,但对其杀死上皮细胞的机制或细胞毒性在细菌存活中所起的作用知之甚少。最近的微生物组研究表明,阴道链球菌和女性生殖道内的人乳头瘤病毒(HPV)之间存在关联,这表明HPV和阴道链球菌可能在这个共享的生态位中以某种方式相互作用。我们分析了来自阴道人类微生物组计划的16S rRNA调查和HPV分型数据,发现与其他报告一致,阴道链球菌与HPV感染有关。为了验证HPV促进阴道弧菌生长的假设,我们定量评估了阴道弧菌与hpv16阳性和HPV阴性的人宫颈角化细胞(HCK)共培养的生长和细胞毒性。通过组织学评估,表达HPV16的器官型HCK筏对阴道链球菌引起的损伤更有抵抗力,并且相对于hpv阴性的HCK筏,支持细菌生长增加。当阴道链球菌与hpv16阳性和hpv阴性的HCK单层共培养时,在hpv16阳性和hpv阴性的细胞中都观察到细胞毒性,但hpv16阳性细胞更能抵抗细菌的毒性作用,并在较长时间内支持细菌生长。综上所述,HPV16可能保护宫颈角化细胞免受阴道链球菌的细胞毒性作用,阻止定植细胞的根除并支持细菌生长,这可能是阴道链球菌与HPV体内关联的基础。阴道芽胞杆菌(S. vaginalis)是一种生活在人类阴道内的细菌,如果它侵入子宫,会在怀孕期间引起并发症。它能够杀死宫颈上皮细胞。人乳头瘤病毒(HPV)是性传播病毒,可导致生殖器病变和宫颈癌。最近,多篇报道描述了阴道链球菌和HPV之间的联系。本研究使用表达高危型HPV、HPV16和HPV阴性细胞的培养宫颈上皮细胞来确定HPV是否促进阴道链球菌的生长。我们发现HPV16促进暴露于阴道链球菌的宫颈上皮细胞的存活。宫颈上皮细胞的存活可能有利于阴道球菌的生长,阴道球菌附着并以这些细胞为食,在女性生殖道中存活。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
mSphere
mSphere Immunology and Microbiology-Microbiology
CiteScore
8.50
自引率
2.10%
发文量
192
审稿时长
11 weeks
期刊介绍: mSphere™ is a multi-disciplinary open-access journal that will focus on rapid publication of fundamental contributions to our understanding of microbiology. Its scope will reflect the immense range of fields within the microbial sciences, creating new opportunities for researchers to share findings that are transforming our understanding of human health and disease, ecosystems, neuroscience, agriculture, energy production, climate change, evolution, biogeochemical cycling, and food and drug production. Submissions will be encouraged of all high-quality work that makes fundamental contributions to our understanding of microbiology. mSphere™ will provide streamlined decisions, while carrying on ASM''s tradition for rigorous peer review.
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