Metallomic characterization of induced periapical lesions—In vivo study

IF 7.1 1区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

International endodontic journal Pub Date : 2025-07-01 DOI:10.1111/iej.14274

Jennifer Santos Pereira, Brenda Fornazaro Moraes, Anna Carolina Neves Leutz, Hellen Carolliny de Souza Nicolau, Rafaela Caires Santos, Talita Tartari, Brenda Paula Figueiredo de Almeida Gomes, Adriana de Jesus Soares, Ana Cristina Padilha Janini, Lauter Eston Pelepenko, Marina Angélica Marciano

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引用次数: 0

Abstract

Aim

Metals are essential for metabolism and play a crucial role in various biological processes. Therefore, the aim of this study was to characterize and compare the metallomic profiles of periapical lesions and healthy alveolar bone in rats using complementary analytical techniques.

Methodology

This study included 76 lower first molars (from 38 Wistar rats) where induced periapical lesions and controls (sham) were compared. Periapical lesion induction was performed by pulp exposure of these teeth, allowing infection development. After 40 days, the animals were reweighed, euthanised and their hemimandibles analysed by periapical radiography, histological analysis, micro-computed tomography (μ-CT), X-ray fluorescence microscopy (μ-XRF), scanning electron microscopy with energy dispersive spectroscopy (SEM/EDS), inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma mass spectrometry (ICP-MS). Ten essential metals for metabolism were analysed (sodium, potassium, magnesium, calcium, iron, manganese, cobalt, copper, zinc and molybdenum). The analyses observed a significance level of 5%.

Results

Radiographic analysis confirmed the induction of periapical lesions, without difference in animal weight between the conditions (p > .05). Histologically, the periapical lesions showed intense inflammatory infiltrate, cells with bluish cytoplasmic granules, alveolar resorption and scores ranging from moderate to intense. The μ-CT analysis of the induced lesion revealed a significant difference in the periapical region volume (12.74 mm³, p = .0017). SEM/EDS showed limited sensitivity for the investigated chemical elements; however, μ-XRF identified diminished intensities for calcium and zinc and increased intensities for iron. ICP-MS and ICP-OES identified increased concentrations of sodium (p = .0137), potassium (p = .0005), calcium (0.0059), magnesium (p = .0004), iron (p < .001), ⁵⁶iron (p = .0078), ⁵⁷iron (p = .0315) and manganese (p < .001) within the induced periapical lesion, suggesting a direct impact on mineral homeostasis following this pathology.

Conclusions

This study demonstrated differences in the levels of various essential elements between conditions with periapical lesions and healthy controls.

Abstract Image

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诱导根尖周围病变的金属学特征-体内研究。

目的：金属是人体代谢的必需物质，在多种生物过程中起着至关重要的作用。因此，本研究的目的是利用互补分析技术表征和比较大鼠根尖周围病变和健康牙槽骨的金属谱。方法：本研究包括76颗下第一磨牙（来自38只Wistar大鼠），其中诱导的根尖周病变与对照组（假）进行比较。根尖周围病变的诱导是通过牙髓暴露这些牙齿，允许感染发展。40 d后，对实验动物进行称重、安乐死，并用根尖周围x线摄影、组织学分析、显微计算机断层扫描（μ-CT）、x射线荧光显微镜（μ-XRF）、扫描电子显微镜（SEM/EDS）、电感耦合等离子体发射光谱（ICP-OES）和电感耦合等离子体质谱（ICP-MS）对实验动物的半下肢进行分析。研究人员分析了新陈代谢所需的十种必需金属（钠、钾、镁、钙、铁、锰、钴、铜、锌和钼）。分析发现显著性水平为5%。结果：x线分析证实了根尖周围病变的诱导，两种情况下动物体重无差异（p < 0.05）。组织学上，根尖周围病变表现为强烈的炎症浸润，细胞呈蓝色细胞质颗粒，肺泡吸收，评分从中等到强烈不等。诱导病变的μ-CT分析显示，在根尖周围区域体积（12.74 mm3, p = 0.0017）上差异有统计学意义。SEM/EDS对所研究的化学元素的敏感性有限；然而，μ-XRF发现钙和锌的强度降低，铁的强度增加。ICP-MS和ICP-OES鉴定出钠（p = 0.0137）、钾（p = 0.0005）、钙（0.0059）、镁（p = 0.0004）、铁（p = 0.0078）、铁（p = 0.0315）和锰(p)浓度升高。结论：本研究表明，在根尖周病变患者和健康对照组之间，各种必需元素的水平存在差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International endodontic journal 医学-牙科与口腔外科

CiteScore

10.20

自引率

28.00%

发文量

195

审稿时长

4-8 weeks

期刊介绍： The International Endodontic Journal is published monthly and strives to publish original articles of the highest quality to disseminate scientific and clinical knowledge; all manuscripts are subjected to peer review. Original scientific articles are published in the areas of biomedical science, applied materials science, bioengineering, epidemiology and social science relevant to endodontic disease and its management, and to the restoration of root-treated teeth. In addition, review articles, reports of clinical cases, book reviews, summaries and abstracts of scientific meetings and news items are accepted. The International Endodontic Journal is essential reading for general dental practitioners, specialist endodontists, research, scientists and dental teachers.