Investigation of Possible Telomerase Reverse Transcriptase Promoter Mutation in Human Chemically Induced Liver Progenitors.

IF 3.9 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Hepatology Research Pub Date : 2025-07-01 DOI:10.1111/hepr.14240

Baglan Askeyev, Akihiko Soyama, Daisuke Miyamoto, Kayo Hasegawa, Hajime Matsushima, Takanobu Hara, Takashi Hamada, Kazushige Migita, Takuro Fujita, Hajime Imamura, Mampei Yamashita, Tomohiko Adachi, Susumu Eguchi

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引用次数: 0

Abstract

Aim: This study aimed to investigate the presence of telomerase reverse transcriptase (TERT) promoter mutations in human chemically induced liver progenitors (hCLiPs) and to evaluate their potential association with carcinogenesis.

Methods: TERT promoter mutations were analyzed in 20 formalin-fixed, paraffin-embedded HCC samples, 19 liver cirrhosis (LC) samples, and five hCLiPs derived from cirrhotic liver tissue. Genomic DNA was extracted, and droplet digital PCR was performed to detect TERT promoter mutations (C228T and C250T).

Results: No TERT promoter mutations were detected in hCLiPs or LC samples. In contrast, 11 of 20 (55%) HCC samples carried the C228T mutation, with mutant allele frequencies ranging from 2.7% to 28.0% (mean ± SD: 21.6 ± 11.05%). The C250T mutation was not identified in any sample.

Conclusion: These findings show that hCLiPs reprogrammed using small molecules do not harbor TERT promoter mutations, supporting their genomic stability and potential safety for clinical applications.

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化学诱导的人肝祖细胞中端粒酶逆转录酶启动子可能突变的研究。

目的：本研究旨在探讨端粒酶逆转录酶（TERT）启动子突变在人类化学诱导的肝祖细胞（hCLiPs）中的存在，并评估其与癌变的潜在关联。方法：对20例福尔马林固定、石蜡包埋的HCC样本、19例肝硬化（LC）样本和5例来自肝硬化肝组织的hCLiPs样本进行TERT启动子突变分析。提取基因组DNA，采用微滴数字PCR检测TERT启动子突变（C228T和C250T）。结果：在hCLiPs和LC样品中未检测到TERT启动子突变。相比之下，20例HCC样本中有11例（55%）携带C228T突变，突变等位基因频率范围为2.7%至28.0%（平均±标准差：21.6±11.05%）。在任何样本中均未发现C250T突变。结论：这些发现表明，使用小分子重编程的hCLiPs不存在TERT启动子突变，支持其基因组稳定性和临床应用的潜在安全性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Hepatology Research 医学-胃肠肝病学

CiteScore

8.30

自引率

14.30%

发文量

124

审稿时长

1 months

期刊介绍： Hepatology Research (formerly International Hepatology Communications) is the official journal of the Japan Society of Hepatology, and publishes original articles, reviews and short comunications dealing with hepatology. Reviews or mini-reviews are especially welcomed from those areas within hepatology undergoing rapid changes. Short communications should contain concise definitive information.