Transcriptomic analysis reveals candidate molecular pathways involved in pea (Pisum sativum L.) resistance to pea aphid (Acyrthosiphon pisum Harris) biotypes.

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Rémi Ollivier, Stéphanie Robin, Marc Galland, Po-Yuan Shih, Stéphanie Morlière, Maria K Paulmann, Jonathan Gershenzon, Grit Kunert, Marie-Laure Pilet-Nayel, Jean-Christophe Simon, Akiko Sugio
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引用次数: 0

Abstract

Pea (Pisum sativum L.) is a major legume crop frequently infested by various parasites, including the pea aphid (Acyrthosiphon pisum Harris), which takes nutrients from its host and transmits phytopathogenic viruses. This aphid species comprises several biotypes, each one capable of feeding and reproducing on a limited range of specific legume species. However, little is known about the transcriptional response of the plants to different aphid biotypes. To investigate pea defense mechanisms against the pea-adapted and pea non-adapted A. pisum biotypes, we analyzed the transcriptional responses by mRNA sequencing of six pea genotypes with contrasting resistance levels and different haplotypes at the ApRVII locus. This locus confers partial resistance to the pea-adapted and non-adapted A. pisum biotypes. Transcriptomic analyses of pea genotypes with and without aphid infestation revealed 9,217 differentially expressed genes in pea genotypes infested with the pea-adapted aphids, while fewer genes, 1,561 genes total, were expressed in response to the pea non-adapted aphids. Both aphid biotypes activated immune responses and the biosynthesis of secondary metabolites, including flavonoids. However, the pea-adapted biotype appeared to suppress multiple pathways associated with photosynthesis, cell wall biosynthesis, fatty acid metabolism, and other growth-related processes. Candidate genes potentially involved in aphid resistance were identified both within and outside of the ApRVII locus. These findings provide insights into pea resistance mechanisms against both pea-adapted and pea non-adapted A. pisum biotypes, as well as the ability of the adapted biotype to modulate pea defenses leading to host susceptibility, and pave the way for follow-up studies, including metabolomic analyses.

转录组学分析揭示了豌豆(Pisum sativum L.)对豌豆蚜虫(Acyrthosiphon Pisum Harris)生物型抗性的候选分子途径。
豌豆(Pisum sativum L.)是一种主要的豆科作物,经常受到各种寄生虫的侵袭,包括豌豆蚜虫(Acyrthosiphon Pisum Harris),它从宿主那里获取营养并传播植物病原病毒。这种蚜虫包括几种生物型,每一种都能在有限范围内的特定豆科植物物种上取食和繁殖。然而,关于植物对不同蚜虫生物型的转录反应知之甚少。为了研究豌豆对豌豆适应型和非适应型豌豆的防御机制,我们通过对6种豌豆基因型的mRNA测序,分析了ApRVII位点上不同单倍型和抗性水平的豌豆的转录反应。该基因座对豌豆适应型和非豌豆适应型具有部分抗性。对受蚜和未受蚜影响的豌豆基因型进行转录组学分析发现,受蚜影响的豌豆基因型中有9217个差异表达基因,而未受蚜影响的豌豆基因型中有1561个差异表达基因。两种蚜虫生物型都激活了免疫反应和次生代谢物的生物合成,包括黄酮类化合物。然而,适应豌豆的生物型似乎抑制了与光合作用、细胞壁生物合成、脂肪酸代谢和其他生长相关过程相关的多种途径。在ApRVII基因座内外均鉴定出可能参与蚜虫抗性的候选基因。这些发现为豌豆对豌豆适应型和豌豆非适应型豌豆的抗性机制提供了见解,以及适应型生物型调节豌豆防御导致寄主敏感性的能力,并为后续研究铺平了道路,包括代谢组学分析。
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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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