Transcriptome analysis of two photo-thermo-sensitive genic male-sterile lines reveal GA-mediated regulatory mechanism for rice panicle exsertion.

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

BMC Genomics Pub Date : 2025-07-01 DOI:10.1186/s12864-025-11809-1

Pengpeng Fang, Yi Zhou, Li Zhang, Wuhan Zhang, Qiang He, Duo Lan, Jia Zeng, Huafeng Deng, Fu Shu, Pingyong Sun

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引用次数: 0

Abstract

Background: Hybrid rice is pivotal for ensuring food security in China, yet challenges persist in its seed production. Sterile lines commonly face issues such as necking, gibberellins (GAs) are key regulators of panicle exsertion in rice, necessitating increased application of GAs during seed production, leading to elevated costs and environmental pollution. The novel photoperiod-thermosensitive genic male sterile line Shuang 1S addresses these limitations through its unique non-necked morphology and has super high yield of seed production.

Results: To elucidate the regulatory pathways governing panicle exsertion and identify novel potential genes, this study assessed endogenous GA levels and performed RNA-seq analysis on two sterile lines, Shuang 1S and Han 2S, which displayed notable differences in panicle exsertion. Among 20 sterile lines examined, Shuang 1S demonstrated the highest panicle grains exsertion rate (91.03%) and the longest inverted first internode (28.24 cm). The findings revealed a 1.5-fold increase in GA content in Shuang 1S compared to Han 2S. Additionally, 2,147 up-regulated and 2,709 down-regulated genes were identified between SP (Shuang 1S) and HP (Han 2S), including 15 genes (10 up-regulated and 5 down-regulated) involved in GA biosynthesis and metabolism. KEGG enrichment analysis of the differentially expressed genes (DEGs) revealed significant enrichment in pathways including diterpenoid biosynthesis and plant hormone signal transduction, which are associated with gibberellic acid (GA) synthesis and catabolism.

Conclusions: We found that panicle exsertion was directly related to GAs content, and mainly enriched in diterpenoid biosynthesis, plant hormone signal transduction. These findings present new potential candidate genes for future investigations into the molecular mechanisms underlying the regulation of panicle exsertion in rice.

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两个光热敏感雄性不育系的转录组分析揭示了ga介导的水稻穗外露调控机制。

背景：杂交水稻是确保中国粮食安全的关键，但其种子生产仍面临挑战。不育品系普遍面临缩颈等问题，赤霉素是水稻穗外露的关键调控因子，需要在制种过程中增加赤霉素的施用，导致成本增加和环境污染。新型光敏性雄性不育系双1S通过其独特的无颈状形态和超高的制种产量解决了这些问题。结果：为了阐明穗外露的调控途径，发现新的潜在基因，本研究对两种不育系双1S和韩2S进行了内源GA水平测定和RNA-seq分析。在所检测的20个不育品系中，双1S穗粒外露率最高（91.03%），第一节间倒立最长（28.24 cm）。结果表明，双1S的GA含量比汉2S增加了1.5倍。在SP（双1S）和HP（汉2S）间共鉴定出2147个上调基因和2709个下调基因，其中参与GA生物合成和代谢的基因15个（上调10个，下调5个）。对差异表达基因（DEGs）的KEGG富集分析显示，与赤霉素（GA）合成和分解代谢相关的二萜类生物合成和植物激素信号转导通路显著富集。结论：我们发现穗部外露与GAs含量直接相关，主要富集于二萜类生物合成和植物激素信号转导。这些发现为进一步研究水稻穗外露调控的分子机制提供了新的潜在候选基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Genomics 生物-生物工程与应用微生物

CiteScore

7.40

自引率

4.50%

发文量

769

审稿时长

6.4 months

期刊介绍： BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.