The promotion of liver vitamin metabolism is of great significance for laying hens during fasting.

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jun Zhang, Zhixuan Luo, Mengyan Zhang, Yujie Gong, Yadong Tian, Ruili Han, Donghua Li, Yujie Guo, Xiangtao Kang, Yawei Yang, Zhuanjian Li, Ruirui Jiang
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引用次数: 0

Abstract

Background: Induced molt is an effective measure to reduce the introduction cost, cope with the continuous rise of feed cost, and realize the prolonged rearing of laying hens as well. Vitamins are beneficial to the antioxidant capacity and reproductive performance of laying hens, however, studies on vitamin metabolism during fasting are rarely reported.

Results: We analyzed the association between cecal metabolome and liver transcriptome of laying hens during molt. The results showed that 3009 differences genes (FDR < 0.05), among which there were 62 differential genes related to vitamin metabolism. Eight core genes (ALDH1A1, CYP1A1, CYP1A4, AOX2P, AOX1, CYP3A7, BCAT1, CYP26B1) were obtained by protein network interaction (PPI). These genes were mainly enriched in Metabolic pathways, Retinol metabolism, Folate biosynthesis, One carbon pool by Folate, and Chemical carcinogenesis. After association analysis between these genes and cecal metabolites, a total of 176 differential metabolites were obtained. Among them, the metabolites with higher connectivity were Bifemelane, L-valine, Butyryl Fentanyl-D5 and Rimcazole.

Conclusion: During fasting, vitamin A and vitamin E stored in the liver of laying hens are released in large quantities with the oxidative decomposition of lipids in the liver during fasting, which accelerates the metabolism of vitamin A in the liver. Folate and biotin may participate in the physiological remodeling of laying hens through epigenetic regulation. In addition, through association analysis, we constructed a data platform for vitamin metabolism-related pathway genes and cecal metabolites, laying a foundation for future research. However, whether the relationship between gene expression in the liver and metabolites in the cecum is bidirectional or unidirectional is still unclear and needs to be further studied.

对蛋鸡禁食期间肝脏维生素代谢的促进具有重要意义。
背景:诱导脱皮是降低引进成本、应对饲料成本持续上涨、实现蛋鸡长期性饲养的有效措施。维生素有利于蛋鸡的抗氧化能力和繁殖性能,但关于禁食期间维生素代谢的研究很少报道。结果:我们分析了产蛋鸡蜕皮过程中盲肠代谢组与肝脏转录组的关系。结论:空腹期间,随着肝脏脂质氧化分解,储存在肝脏中的维生素A和维生素E被大量释放,加速了肝脏中维生素A的代谢。叶酸和生物素可能通过表观遗传调控参与了蛋鸡的生理重塑。此外,通过关联分析,我们构建了维生素代谢相关途径基因与盲肠代谢物的数据平台,为今后的研究奠定基础。然而,肝脏中基因表达与盲肠代谢物之间的关系是双向的还是单向的,尚不清楚,需要进一步研究。
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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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