Screening of Bioactive Fractions from Balanites aegyptiaca and Pterocarpus Marsupium for Anticancer Effects in HepG2 and U87MG Cels.

IF 3 4区医学 Q3 CHEMISTRY, MEDICINAL

Anti-cancer agents in medicinal chemistry Pub Date : 2025-06-23 DOI:10.2174/0118715206396025250614030603

Divya Vashishth, Mansi Yadav, Ajay Kumar, Gulshan Rohilla, Minakshi Vashist, Sudhir Kumar Kataria

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引用次数: 0

Abstract

Introduction: Cancer is a group of diseases caused by uncontrollable cell growth. Herbal medicines, derived from plants, have been used for centuries across cultures for their therapeutic benefits, effectively treating conditions like cancer. This study represents the anticancer effects of fractions of some medicinal plant extracts along with their apoptotic studies and their induction through p53-mediated Bax and Bcl-2 mRNA expression in HepG2 and U87MG cells.

Methods: The fractionation of crude methanolic extracts was done using Column Chromatography and Thin Layer Chromatography. The fractions were analysed for cytotoxicity against both the cell lines by MTT assay. Cancer cells were treated with 2 most active fractions and their mechanism of apoptosis induction was assessed by Flow Cytometry studies and the mRNA expression levels of p53, Bax, and Bcl-2 were determined by Reverse Transcriptase PCR. The presence of phytoconstituents in the active fractions was analysed by GC-MS.

Results: The active fractions revealed the apoptosis induction in both the cell lines and the RT-PCR studies suggested the mechanism of apoptosis induction through upregulation of p53 and Bax and downregulation of Bcl-2 mRNA. The GC-MS analysis of active fractions from Balanites aegyptiaca and Pterocarpus marsupium revealed the presence of phytochemicals such as 4-O-Methylmannose, Oleic acid, Erucic acid, etc. which might have contributed to the anti-proliferative and apoptotic effects of these fractions.

Discussion: 4-O-Methylmannose was the major component identified with the highest peak area of 59%. The fractions from all the 4 plant extracts demonstrated significant cytotoxic effects on the liver (HepG2) and brain (U87MG) cancer cell lines, with particular emphasis on the active fractions BA FII, PM FII, and PM FIII. Additionally, the mechanisms of apoptosis induction through the modulation of p53, Bax, and Bcl-2 pathways, along with the presence of bioactive compounds further support the anticancer efficacy of these plant extracts. Also, to the best of our knowledge, this is the first study on fractions of Balanites aegyptiaca and Pterocarpus marsupium against U87MG cells.

Conclusion: The results highlight the promising potential of plant-derived natural products as anticancer agents. These findings provide valuable insight into the potential of herbal medicines and encourage further exploration of plant-based therapies for cancer treatment.

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埃及巴兰和有袋翼柏抗HepG2和U87MG细胞活性组分的筛选

癌症是一组由于细胞生长不可控而引起的疾病。几个世纪以来，从植物中提取的草药一直被各种文化所使用，因为它们具有治疗功效，能有效地治疗癌症等疾病。本研究研究了部分药用植物提取物的抗癌作用，以及它们通过p53介导的Bax和Bcl-2 mRNA表达诱导HepG2和U87MG细胞凋亡的研究。方法：采用柱层析和薄层析对粗甲醇提取物进行分离。用MTT法分析各组分对两种细胞系的细胞毒性。用2种活性组分处理肿瘤细胞，流式细胞术检测其诱导凋亡机制，逆转录酶PCR检测p53、Bax、Bcl-2 mRNA表达水平。采用气相色谱-质谱法分析了活性组分中植物成分的存在。结果：活性组分对两种细胞系均有诱导凋亡的作用，RT-PCR研究提示其诱导凋亡的机制是通过上调p53和Bax，下调Bcl-2 mRNA。对埃及巴兰和有袋翼果的活性组分进行气相色谱-质谱分析，发现其含有4- o -甲基甘露糖、油酸、芥酸等植物化学物质，可能与抗细胞增殖和细胞凋亡作用有关。讨论：4- o -甲基甘露糖为主要成分，峰面积最高达59%。4种植物提取物对肝癌细胞（HepG2）和脑癌细胞（U87MG）均有显著的细胞毒作用，其中活性组分BA FII、PM FII和PM FIII的作用尤为显著。此外，通过调节p53、Bax和Bcl-2途径诱导细胞凋亡的机制，以及生物活性化合物的存在，进一步支持了这些植物提取物的抗癌功效。此外，据我们所知，这是第一次研究巴兰（Balanites aegyptica）和有袋翼龙（Pterocarpus marsupium）对U87MG细胞的作用。结论：植物源性天然产物具有良好的抗癌潜力。这些发现为草药的潜力提供了宝贵的见解，并鼓励进一步探索以植物为基础的癌症治疗方法。

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来源期刊

Anti-cancer agents in medicinal chemistry ONCOLOGY-CHEMISTRY, MEDICINAL

CiteScore

5.10

自引率

3.60%

发文量

323

审稿时长

4-8 weeks

期刊介绍： Formerly: Current Medicinal Chemistry - Anti-Cancer Agents. Anti-Cancer Agents in Medicinal Chemistry aims to cover all the latest and outstanding developments in medicinal chemistry and rational drug design for the discovery of anti-cancer agents. Each issue contains a series of timely in-depth reviews and guest edited issues written by leaders in the field covering a range of current topics in cancer medicinal chemistry. The journal only considers high quality research papers for publication. Anti-Cancer Agents in Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments in cancer drug discovery.