Different Circulating Endothelial Microvesicle Subtype Signature in Subacute and Chronic Spinal Cord Injury.

IF 2.4 Q1 REHABILITATION

Topics in Spinal Cord Injury Rehabilitation Pub Date : 2025-01-01 Epub Date: 2025-06-19 DOI:10.46292/sci24-00068

Andrew J Park, Hannah K Fandl, Vinicius P Garcia, Auburn R Berry, Kendra N Wegerson, Emily I Ostrander, Hannah L Cardenas, Noah M DeSouza, Jared J Greiner, Brian Stauffer, Christopher A DeSouza

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Abstract

Objectives: The aim of this study was to determine whether circulating concentrations of activation- and apoptosis-derived endothelial cell-derived microvesicles (EMVs) differ between adults after subacute (time since injury ≤6 months) and chronic (time since injury >12 months) spinal cord injury (SCI).

Methods: Peripheral blood was collected from 43 adults (age range 18-71 years): 12 non-injured adults (9 male/3 female), 16 adults with subacute cervical and high thoracic (C2-T3) motor complete injuries (13 male/3 female; time since injury 1-3 months), and 15 adults with chronic cervical and high thoracic (C1-T2) motor complete injuries (14 male/1 female; time since injury 12-52 months). EMVs were defined by markers of endothelial origin either by activation (CD62e⁺) or apoptosis (CD31⁺/CD42b^-) by flow cytometry. Activation-derived but not apoptosis-derived EMVs were significantly higher (P < .05) in adults with chronic SCI (median [IQR], 139 [83-181] EMVs/μL) compared with adults with subacute SCI (median [IQR], 99 [83-104] EMVs/μL) and non-injured adults (median [IQR], 74 [51-104] EMVs/μL). In contrast, apoptosis-derived but not activation-derived EMVs were significantly higher (P < .05) in adults with subacute SCI (mean ± SD, 77 ± 17 EMVs/μL) compared with adults with chronic SCI (mean ± SD, 55 ± 19 EMVs/μL) and non-injured adults (mean ± SD, 52 ± 25 EMVs/μL). Differential expression of circulating EMVs in adults with SCI during the subacute and chronic phase of injury may represent a biomarker of the vascular environment associated with each condition. Our findings suggest that the vascular phenotype is markedly different in subacute compared with the chronic SCI and provide insight into endothelial function after SCI.

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亚急性和慢性脊髓损伤的不同循环内皮微泡亚型特征。

目的：本研究的目的是确定亚急性（损伤后≤6个月）和慢性（损伤后≤12个月）脊髓损伤（SCI）后成人中活化和凋亡来源的内皮细胞来源的微囊泡（emv）的循环浓度是否存在差异。方法：收集43例成人（年龄18-71岁）外周血：12例未损伤成人（男9例/女3例），16例亚急性颈、胸高位（C2-T3）运动完全性损伤成人(男13例/女3例；15例成人慢性颈、胸高位（C1-T2）运动完全性损伤(男14 /女1；受伤后12-52个月)。emv由内皮来源的标记物定义，通过流式细胞术通过激活（CD62e+）或凋亡（CD31+/CD42b-）。慢性脊髓损伤成人（中位数[IQR]， 139 [83-181] emv /μL）与亚急性脊髓损伤成人（中位数[IQR]， 99 [83-104] emv /μL）和非损伤成人（中位数[IQR]， 74 [51-104] emv /μL）相比，激活衍生而非凋亡衍生的emv显著升高（P < 0.05）。相比之下，与慢性SCI（平均±SD， 55±19 emv /μL）和非损伤成人（平均±SD， 52±25 emv /μL）相比，亚急性SCI成人（平均±SD， 77±17 emv /μL）中凋亡衍生而非激活衍生的emv显著升高（P < 0.05）。成人脊髓损伤亚急性期和慢性期循环emv的差异表达可能代表了与每种情况相关的血管环境的生物标志物。我们的研究结果表明，与慢性脊髓损伤相比，亚急性期的血管表型明显不同，并为脊髓损伤后的内皮功能提供了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Topics in Spinal Cord Injury Rehabilitation REHABILITATION-

CiteScore

3.20

自引率

3.40%

发文量

期刊介绍： Now in our 22nd year as the leading interdisciplinary journal of SCI rehabilitation techniques and care. TSCIR is peer-reviewed, practical, and features one key topic per issue. Published topics include: mobility, sexuality, genitourinary, functional assessment, skin care, psychosocial, high tetraplegia, physical activity, pediatric, FES, sci/tbi, electronic medicine, orthotics, secondary conditions, research, aging, legal issues, women & sci, pain, environmental effects, life care planning