Novel reporter systems to detect cold and osmotic stress responses.

IF 1.3 Q3 BIOCHEMICAL RESEARCH METHODS
Biology Methods and Protocols Pub Date : 2025-06-14 eCollection Date: 2025-01-01 DOI:10.1093/biomethods/bpaf048
Kanon Maruyama, Hodaka Fujii
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引用次数: 0

Abstract

Cells respond to environmental stresses such as cold and osmotic stresses. These stresses induce signal transduction pathways in cells. However, the molecular mechanisms activated by cold and osmotic stresses in higher eukaryotes remain elusive. Previously, we described a reporter system utilizing inducible translocation trap that detects nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase (KBL) in response to cold and osmotic stresses. In the present study, we developed additional reporter systems to detect intracellular events induced by these stresses. These reporter systems will be instrumental to elucidate the intracellular signaling mechanisms activated by these stresses.

新型报告系统检测冷和渗透应激反应。
细胞对环境压力,如寒冷和渗透压力作出反应。这些应激诱导细胞内的信号转导通路。然而,在高等真核生物中,冷胁迫和渗透胁迫激活的分子机制仍然是难以捉摸的。之前,我们描述了一个利用诱导型易位陷阱的报告系统,该系统可以检测2-氨基-3-酮丁酸辅酶a连接酶(KBL)在寒冷和渗透胁迫下的核易位。在目前的研究中,我们开发了额外的报告系统来检测这些应激诱导的细胞内事件。这些报告系统将有助于阐明这些应激激活的细胞内信号机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biology Methods and Protocols
Biology Methods and Protocols Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
3.80
自引率
2.80%
发文量
28
审稿时长
19 weeks
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