Evidence of postnatal neglect-dependent resiliency in thymic, gut, and behavioral outcomes in a mouse model of early life stress

Abstract

Background

Early life is an impressionable period often regarded as the window of opportunity. Environmental exposures, such as stress, in the early postnatal period can influence developmental trajectory and long-term health. The brain and immune systems continue to develop after birth and are shaped by postnatal exposures. While chronic traumatic stress is understood to adversely affect individuals by overwhelming coping abilities, there is evidence for stress exposure to be beneficial by promoting resilience. Early life stress (ELS) is a significant postnatal exposure, which encompasses childhood maltreatment and trauma. Neglect is the most common form of maltreatment in children and takes many forms, including physical and nutritional.

Methods

Here, we describe a novel mouse model of neglect-related ELS based on maternal separation with early weaning (MSEW) with a distinct early weaning (EW) stress group to study how an environment of neglect impacts the developing microbiome, thymocytes, and stress-related behavior. Neglect-related stress was emulated based on scheduled dam-pup separation (physical neglect) and a high carbohydrate early-wean diet (malnutrition). C57BL/6J mice were bred in-house and ELS pups were subjected to: (1) daily dam-pup separation on postnatal days (PD) 2–13 and/or (2) early weaning to a high carbohydrate diet on PD14-21.

Results

The present study focused on a defined early life window (PD0-21) and revealed that MSEW versus EW exposures generate distinguishable and distinct effects on behavior and thymic T cells, leading to phenotypes of stress resilience versus vulnerability. Although impacts of the two ELS groups were indistinguishable on lower gastrointestinal tract microbiome composition, the effects on ELS groups were significant compared to controls.

Conclusions

Our findings provide evidence for circumstances where prior stress can induce resilience and emphasizes the nuanced approach required for studies to begin parsing out toxic versus beneficial stress.