Taurine and Neuroprotection: A Potential Shield Against Methylglyoxal-Induced Toxicity in SH-SY5Y Cells

IF 3.2 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Biochemical and Molecular Toxicology Pub Date : 2025-06-30 DOI:10.1002/jbt.70343

Norah A. Althobaiti, Ifat Alsharif, Reem Hasaballah Alhasani, Aishah E. Albalawi, Mohammad Y. Alshahrani, Amany I. Almars, Ammar A. Basabrain, Mohammad H. Alhashmi, Zahrah R. Alrayes, Hailah M. Almohaimeed, Mona H. Soliman

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引用次数: 0

Abstract

Methylglyoxal (MG) is thought to have harmful effects on Alzheimer's disease (AD). On the other hand, taurine demonstrates promising potential for treating AD. Therefore, we examined the neuroprotective properties of taurine against MG in the SH-SY5Y cells. We used the MTT assay to evaluate the effects of taurine (0.5, 1, and 1.5 mg/mL) and MG (1200 and 2400 μM) on SH-SY5Y cell viability. We measured the expression of interleukin (IL)-6, IL-17, and IL-1β in the target cell line after treatment with MG and taurine. Following the treatment of SH-SY5Y cells with MG and taurine, we evaluated the expression levels of microRNA (miRNA)-101a, miRNA-137, miRNA-222, and miRNA-29c genes using real-time PCR. Furthermore, the Neurogenesis Plus RT² Profiler PCR array was utilized to identify the expression of genes associated with neurogenesis. The survival results indicated that increasing taurine concentrations reduces the toxicity of MG in SH-SY5Y cells. Treatment of SH-SY5Y cells with taurine + MG decreased the expression levels of IL-6, IL-17, and IL-1β compared to those treated with MG (p < 0.05). Treatment with taurine led to increased expression of miRNA-101a, miRNA-137, miRNA-222, and miRNA-29c, with the highest levels observed at a concentration of 1 mg/mL (p < 0.001). The results showed that when SH-SY5Y cells are exposed to MG (2400 μM) + taurine (1.5 mg/mL), genes SHH, BMP2, ERBB2, NEUROG2, BDNF, POU3F3, PARD3, PAX3, NR2E3, NRP2, CXCL1, and EGF had a significant increase (p < 0.005). Taurine protects SH-SY5Y cells from MG-induced toxicity by enhancing cell viability, reducing inflammation, upregulating genes associated with neurogenesis, and upregulating specific miRNAs. This suggests taurine's potential as a therapeutic agent for conditions like AD. However, further in vivo studies and clinical trials are necessary to validate the therapeutic potential of taurine for AD.

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牛磺酸和神经保护：对甲基乙二醛诱导的SH-SY5Y细胞毒性的潜在保护

甲基乙二醛（MG）被认为对阿尔茨海默病（AD）有有害影响。另一方面，牛磺酸显示出治疗阿尔茨海默病的良好潜力。因此，我们研究了牛磺酸对SH-SY5Y细胞中MG的神经保护作用。我们采用MTT法评估牛磺酸（0.5、1和1.5 mg/mL）和mg（1200和2400 μM）对SH-SY5Y细胞活力的影响。我们检测了MG和牛磺酸处理后靶细胞株中白细胞介素(IL)-6、IL-17和IL-1β的表达。在MG和牛磺酸处理SH-SY5Y细胞后，我们使用实时荧光定量PCR技术评估了microRNA (miRNA)-101a、miRNA-137、miRNA-222和miRNA-29c基因的表达水平。此外，利用Neurogenesis Plus RT²Profiler PCR阵列鉴定神经发生相关基因的表达。存活结果表明，增加牛磺酸浓度可降低MG对SH-SY5Y细胞的毒性。与MG组相比，牛磺酸+ MG组SH-SY5Y细胞IL-6、IL-17和IL-1β的表达水平降低（p < 0.05）。牛磺酸处理导致miRNA-101a、miRNA-137、miRNA-222和miRNA-29c的表达增加，在浓度为1mg /mL时达到最高水平（p < 0.001）。结果表明，当SH-SY5Y细胞暴露于MG (2400 μM) +牛磺酸（1.5 MG /mL）时，SHH、BMP2、ERBB2、NEUROG2、BDNF、POU3F3、par3、PAX3、NR2E3、NRP2、CXCL1和EGF基因显著升高（p < 0.005）。牛磺酸通过增强细胞活力、减少炎症、上调与神经发生相关的基因和上调特异性mirna，保护SH-SY5Y细胞免受mg诱导的毒性。这表明牛磺酸有可能成为治疗阿尔茨海默病等疾病的药物。然而，需要进一步的体内研究和临床试验来验证牛磺酸对AD的治疗潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biochemical and Molecular Toxicology 生物-毒理学

CiteScore

5.80

自引率

2.80%

发文量

277

审稿时长

6-12 weeks

期刊介绍： The Journal of Biochemical and Molecular Toxicology is an international journal that contains original research papers, rapid communications, mini-reviews, and book reviews, all focusing on the molecular mechanisms of action and detoxication of exogenous and endogenous chemicals and toxic agents. The scope includes effects on the organism at all stages of development, on organ systems, tissues, and cells as well as on enzymes, receptors, hormones, and genes. The biochemical and molecular aspects of uptake, transport, storage, excretion, lactivation and detoxication of drugs, agricultural, industrial and environmental chemicals, natural products and food additives are all subjects suitable for publication. Of particular interest are aspects of molecular biology related to biochemical toxicology. These include studies of the expression of genes related to detoxication and activation enzymes, toxicants with modes of action involving effects on nucleic acids, gene expression and protein synthesis, and the toxicity of products derived from biotechnology.