Particulate Matter 2.5 Induces FGFR1-mediated Integrin Switch to Promote Non-small Cell Lung Cancer Metastasis.

IF 2.6 4区 医学 Q2 GENETICS & HEREDITY
Nithikoon Aksorn, Zin Zin Ei, Korrakod Petsri, Narumon Phaonakrop, Chalermpong Saenjum, Sittiruk Roytrakul, Pithi Chanvorachote
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引用次数: 0

Abstract

Background/aim: Particulate matter 2.5 (PM2.5) is known to adversely affect human health. While its involvement in lung cancer pathogenesis is recognized, its specific impact on metastasis-related behaviors of lung cancer cells remains largely unexplored.

Materials and methods: In this study, we employed cell culture models, proteomic analysis, and bioinformatic analysis. Target proteins and signaling pathways were validated using western blotting and immunofluorescence assay. Wound healing, transwell migration and phalloidin-rhodamine assays were used to determine the migratory activity.

Results: Proteomic analysis identified 3,795 proteins in both control and PM2.5-treated groups. Among these, proteins associated with metastasis, particularly those related to "cell migration" (GO: 0016477), were highlighted, identifying six key proteins involved in cancer metastasis. Protein-protein interaction analysis pinpointed fibroblast growth factor receptor 1 (FGFR1) as a central target influenced by PM2.5, which promoted cell migration via the Rap1 signaling pathway through integrin signaling. The enhanced migratory behavior of PM2.5-treated cells aligned with proteomic findings, demonstrating that PM2.5 exposure increases the motility of lung cancer cells. Western blotting and immunofluorescence confirmed that PM2.5 exposure led to up-regulation of FGFR1, integrin αV, β1, and activated p-Akt. Notably, PM2.5-treated cells exhibited significantly increased motility and a higher number of filopodia per cell.

Conclusion: These results indicate that FGFR1 is a crucial target in PM2.5-induced metastasis in lung cancer cells, operating through an FGFR1/integrin/Akt signaling axis. This study advances our understanding of the role of PM2.5 in lung cancer metastasis and suggests potential therapeutic strategies to mitigate cancer progression.

颗粒物质2.5诱导fgfr1介导的整合素开关促进非小细胞肺癌转移
背景/目的:已知颗粒物2.5 (PM2.5)对人类健康有不利影响。虽然它在肺癌发病机制中的作用已被确认,但其对肺癌细胞转移相关行为的具体影响仍未被广泛探索。材料和方法:本研究采用细胞培养模型、蛋白质组学分析和生物信息学分析。利用western blotting和免疫荧光法验证靶蛋白和信号通路。伤口愈合、跨井迁移和phalloidin-rhodamine测定迁移活性。结果:蛋白质组学分析在对照组和pm2.5处理组中鉴定了3795个蛋白质。其中,与转移相关的蛋白,特别是与“细胞迁移”相关的蛋白(GO: 0016477)被强调,确定了6个参与癌症转移的关键蛋白。蛋白-蛋白相互作用分析确定成纤维细胞生长因子受体1 (FGFR1)是受PM2.5影响的中心靶点,它通过整合素信号通路通过Rap1信号通路促进细胞迁移。PM2.5处理细胞的迁移行为增强与蛋白质组学研究结果一致,表明PM2.5暴露增加了肺癌细胞的运动性。Western blot和免疫荧光证实PM2.5暴露导致FGFR1、整合素αV、β1上调,p-Akt活化。值得注意的是,pm2.5处理的细胞表现出显著增加的运动性和每个细胞丝状足的数量。结论:这些结果表明FGFR1是pm2.5诱导的肺癌细胞转移的关键靶点,通过FGFR1/整合素/Akt信号轴起作用。这项研究促进了我们对PM2.5在肺癌转移中的作用的理解,并提出了减缓癌症进展的潜在治疗策略。
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来源期刊
Cancer Genomics & Proteomics
Cancer Genomics & Proteomics ONCOLOGY-GENETICS & HEREDITY
CiteScore
5.00
自引率
8.00%
发文量
51
期刊介绍: Cancer Genomics & Proteomics (CGP) is an international peer-reviewed journal designed to publish rapidly high quality articles and reviews on the application of genomic and proteomic technology to basic, experimental and clinical cancer research. In this site you may find information concerning the editorial board, editorial policy, issue contents, subscriptions, submission of manuscripts and advertising. The first issue of CGP circulated in January 2004. Cancer Genomics & Proteomics is a journal of the International Institute of Anticancer Research. From January 2013 CGP is converted to an online-only open access journal. Cancer Genomics & Proteomics supports (a) the aims and the research projects of the INTERNATIONAL INSTITUTE OF ANTICANCER RESEARCH and (b) the organization of the INTERNATIONAL CONFERENCES OF ANTICANCER RESEARCH.
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