Noggin Promotes Cell Proliferation Through Up-regulating EGFR/HER2 in Pancreatic Cancer Cells.

IF 2.6 4区 医学 Q2 GENETICS & HEREDITY
Ming Liu, Karl Chan, Amy Bancroft, Fiona Ruge, Chunyi Hao, Wen G Jiang, Lin Ye
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引用次数: 0

Abstract

Background/aim: Noggin is a secreted antagonist of bone morphogenetic proteins (BMPs) and plays a key role in regulating various developmental and homeostatic biological processes. BMPs have been linked to the development of several types of cancers. However, the impact of Noggin on cellular functions and its role in pancreatic cancer remain unclear. This study aimed to investigate the role of Noggin in pancreatic cancer and its underlying molecular mechanisms.

Materials and methods: Noggin expression in both normal and cancerous pancreatic tissues was assessed using both quantitative and conventional PCR methods, alongside an analysis of publicly available gene expression array datasets. Correlations between Noggin expression and patient survival, TNM staging, tumor/stroma subtypes, and the expression of other cancer-related genes were examined. The influence of Noggin on cellular functions was evaluated in pancreatic cancer cell lines Mia PaCa-2 and PANC-1, which were genetically modified to overexpress Noggin.

Results: Noggin expression was found to be significantly higher in tumor tissues compared to normal pancreatic tissues. Elevated Noggin expression was associated with shorter overall survival in patients. Overexpression of Noggin led to increased proliferation of pancreatic cancer cells. Furthermore, elevated levels of EGFR and HER2 proteins were observed in the PANC-1 and Mia PaCa-2 cell lines, respectively. Treatment with EGFR and HER2 inhibitors reduced Noggin-induced proliferation in these cell lines.

Conclusion: Noggin is overexpressed in pancreatic cancer tissues and is linked to poor patient survival. Noggin promotes the proliferation of pancreatic cancer cells by up-regulating EGFR and HER2.

Noggin通过上调胰腺癌细胞EGFR/HER2促进细胞增殖。
背景/目的:Noggin是骨形态发生蛋白(BMPs)的分泌拮抗剂,在调节多种发育和体内平衡生物学过程中起关键作用。bmp与几种癌症的发生有关。然而,Noggin对细胞功能的影响及其在胰腺癌中的作用尚不清楚。本研究旨在探讨Noggin在胰腺癌中的作用及其潜在的分子机制。材料和方法:使用定量和常规PCR方法评估正常和癌性胰腺组织中Noggin的表达,同时分析公开可用的基因表达阵列数据集。检测Noggin表达与患者生存、TNM分期、肿瘤/基质亚型及其他癌症相关基因表达的相关性。在经过基因修饰过表达Noggin的胰腺癌细胞系Mia PaCa-2和PANC-1中,研究了Noggin对细胞功能的影响。结果:肿瘤组织中Noggin的表达明显高于正常胰腺组织。Noggin表达升高与患者总生存期缩短相关。Noggin的过度表达导致胰腺癌细胞增殖增加。此外,在PANC-1和Mia PaCa-2细胞系中分别观察到EGFR和HER2蛋白水平升高。用EGFR和HER2抑制剂治疗可减少noggin诱导的这些细胞系的增殖。结论:Noggin在胰腺癌组织中过度表达,与患者生存率低有关。Noggin通过上调EGFR和HER2促进胰腺癌细胞的增殖。
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来源期刊
Cancer Genomics & Proteomics
Cancer Genomics & Proteomics ONCOLOGY-GENETICS & HEREDITY
CiteScore
5.00
自引率
8.00%
发文量
51
期刊介绍: Cancer Genomics & Proteomics (CGP) is an international peer-reviewed journal designed to publish rapidly high quality articles and reviews on the application of genomic and proteomic technology to basic, experimental and clinical cancer research. In this site you may find information concerning the editorial board, editorial policy, issue contents, subscriptions, submission of manuscripts and advertising. The first issue of CGP circulated in January 2004. Cancer Genomics & Proteomics is a journal of the International Institute of Anticancer Research. From January 2013 CGP is converted to an online-only open access journal. Cancer Genomics & Proteomics supports (a) the aims and the research projects of the INTERNATIONAL INSTITUTE OF ANTICANCER RESEARCH and (b) the organization of the INTERNATIONAL CONFERENCES OF ANTICANCER RESEARCH.
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