Expression analysis of microRNA and lncRNA in visceral adipose tissue of obese and non-obese individuals.

IF 0.9 Q3 AGRICULTURE, MULTIDISCIPLINARY
А Бейркдар, Д Е Иванощук, О В Тузовская, Н С Широкова, Е В Каштанова, Я В Полонская, Ю И Рагино, Е В Шахтшнейдер
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引用次数: 0

Abstract

Long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) play important roles in all biological processes, including adipogenesis, lipid metabolism, and insulin response. Analyzing expression patterns of lncRNAs and miRNAs in human visceral fat tissue can enhance our understanding of their roles in metabolic disorders. Our research aims to investigate the expression of lncRNAs (ASMER1, SNHG9, P5549, P19461, and GAS5) and miRNAs (miR-26A, miR-222, miR-221, and miR-155) in visceral adipose tissues of individuals with abdominal obesity (n = 70) compared to their levels in non-obese participants (n = 31), using Real-Time PCR. Among the tested miRNAs, only miR-26A was significantly downregulated in the visceral adipose tissue of obese individuals, with no significant change in the expression of miR- 26A in obese people with or without type 2 diabetes. Similarly, of the tested lncRNAs, only GAS5 showed significantly higher expression levels in obese patients with type 2 diabetes (T2D) (n = 10) compared to obese patients without T2D (n = 60). To test possible interactions between the analyzed non-coding RNAs, we used Spearman's bivariate correlation test. GAS5 expression levels showed a weak negative correlation (p < 0.05, rs = 0.25) with miR-155 levels in obese patients only. Conversely, a strong positive correlation (p <0.01, rs = 0.92) between SNHG9 and GAS5 was found in the non-obese group, with a weaker correlation in abdominally obese patients (p < 0.01, rs = 0.67); additionally, miR-26A and miR-155 levels were moderately correlated in the non-obese group (p < 0.05, rs = 0.47) and were found to correlate weakly in obese patients (p < 0.05, rs = 0.26). Our results showed that abdominally obese participants demonstrated higher expression levels of miR-26A in visceral adipose tissue and a significantly lower correlation between GAS5 and SNHG9 expression when compared to non-obese subjects.

肥胖者和非肥胖者内脏脂肪组织中microRNA和lncRNA的表达分析。
长链非编码rna (lncRNAs)和microRNAs (miRNAs)在包括脂肪形成、脂质代谢和胰岛素反应在内的所有生物过程中发挥着重要作用。分析lncrna和mirna在人内脏脂肪组织中的表达模式,可以增强我们对它们在代谢紊乱中的作用的理解。我们的研究旨在利用Real-Time PCR研究腹部肥胖个体(n = 70)内脏脂肪组织中lncRNAs (ASMER1、SNHG9、P5549、P19461和GAS5)和miRNAs (miR-26A、miR-222、miR-221和miR-155)与非肥胖参与者(n = 31)的表达水平。在测试的mirna中,只有miR-26A在肥胖个体的内脏脂肪组织中显著下调,miR-26A在伴有或不伴有2型糖尿病的肥胖人群中的表达没有显著变化。同样,在测试的lncRNAs中,只有GAS5在肥胖合并2型糖尿病(T2D)患者(n = 10)中的表达水平明显高于无T2D的肥胖患者(n = 60)。为了测试分析的非编码rna之间可能的相互作用,我们使用了Spearman的双变量相关检验。仅在肥胖患者中,GAS5表达水平与miR-155水平呈弱负相关(p < 0.05, rs = 0.25)。相反,两者之间存在很强的正相关关系(p
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来源期刊
Vavilovskii Zhurnal Genetiki i Selektsii
Vavilovskii Zhurnal Genetiki i Selektsii AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
1.90
自引率
0.00%
发文量
119
审稿时长
8 weeks
期刊介绍: The "Vavilov Journal of genetics and breeding" publishes original research and review articles in all key areas of modern plant, animal and human genetics, genomics, bioinformatics and biotechnology. One of the main objectives of the journal is integration of theoretical and applied research in the field of genetics. Special attention is paid to the most topical areas in modern genetics dealing with global concerns such as food security and human health.
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