Trauma and Hemorrhage Lead to an Elevation in Fecal Short-Chain Fatty Acids.

IF 2.7 3区医学 Q2 CRITICAL CARE MEDICINE

SHOCK Pub Date : 2025-06-26 DOI:10.1097/SHK.0000000000002645

Daniel N Darlington, Reese B Berger, Jeffrey D Keesee, Susannah E Nicholson, Xiaowu Wu

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引用次数: 0

Abstract

Introduction: Severe trauma and hemorrhage in rats lead to changes in the beta diversity of the commensal bacteria found in the gut. Because Short-Chain Fatty Acids (SCFA) are produced by these bacteria, SCFA concentration may also change following trauma and hemorrhage and reflect these alterations in the microbiome.

Objective: To determine whether changes in SCFA occur after trauma and hemorrhage in the feces and plasma of rodents.

Materials and methods: Polytrauma was induced in isoflurane-anesthetized Sprague-Dawley rats by damage to the small intestine, liver, right leg skeletal muscle, and femur, followed by 20% hemorrhage. Whole blood resuscitation was performed at 1 hour (20%). Rats were euthanized at 2 hours and feces and plasma were analyzed for short-chain fatty acids (SCFA) by liquid chromatography tandem mass spectroscopy.

Results: Of twenty-one SCFA analyzed in the feces and plasma, 11 were measurable. In feces, five demonstrated a significant elevation after 2 hours of severe trauma and hemorrhage (n-8) including propionic (37775 ± 8919 vs. 146591 ± 46734 nM/mg protein: mean ± SEM), pentanoic (10975 ± 2981 vs. 41828 ± 10645), 2-methyl propionic (2621 ± 523 vs. 13798 vs. 2083), 4-methyl pentanoic (1134 ± 302 vs. 4320 ± 1029) and 3-phenyl propionic acid (42194 ± 4863 vs. 153024 ± 38473). The addition of whole blood resuscitation did not change these responses, but led to an additional significant elevation in butyric (68551 ± 10786 vs. 369951 ± 79515) and hexanoic acid (24548 ± 6791 vs. 102002 ± 32069). There was no change in SCFA after trauma, hemorrhage or resuscitation in the plasma (n = 6).

Conclusion: Two hours of severe trauma and hemorrhage lead an elevation in many SCFAs in rat feces. No change in SCFA was found in plasma. Because SCFA are primarily derived from commensal bacteria in the gut, these data suggest that the measurement of SCFA could be used as an index of changes in the gut microbiome in pathological condition including trauma and hemorrhage.

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外伤和出血导致粪便短链脂肪酸升高。

导读：大鼠的严重创伤和出血导致肠道中发现的共生菌的β多样性发生变化。由于短链脂肪酸（SCFA）是由这些细菌产生的，SCFA浓度也可能在创伤和出血后发生变化，并反映了微生物组的这些变化。目的：探讨创伤出血后啮齿动物粪便和血浆中SCFA的变化。材料与方法：异氟醚麻醉大鼠小肠、肝脏、右腿骨骼肌、股骨损伤，并发20%出血。1小时全血复苏（20%）。大鼠于2小时安乐死，用液相色谱串联质谱法分析粪便和血浆中的短链脂肪酸（SCFA）。结果：粪便和血浆中21种SCFA， 11种可测。在粪便中，5例在严重创伤和出血2小时后出现显著升高（n-8），包括丙酸（37775±8919比146591±46734 nM/mg蛋白：平均值±SEM）、戊酸（10975±2981比41828±10645）、2-甲基丙酸（2621±523比13798比2083）、4-甲基戊酸（1134±302比4320±1029）和3-苯基丙酸（42194±4863比153024±38473）。添加全血复苏并没有改变这些反应，但导致丁酸（68551±10786 vs. 369951±79515）和己酸（24548±6791 vs. 102002±32069）的额外显著升高。创伤、出血或复苏后血浆中SCFA无变化（n = 6）。结论：2小时的严重外伤和出血导致大鼠粪便中许多SCFAs升高。血浆中SCFA未见变化。由于SCFA主要来源于肠道中的共生菌，这些数据表明，SCFA的测量可以作为病理状态下（包括创伤和出血）肠道微生物组变化的指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

SHOCK 医学-外科

CiteScore

6.20

自引率

3.20%

发文量

199

审稿时长

1 months

期刊介绍： SHOCK®: Injury, Inflammation, and Sepsis: Laboratory and Clinical Approaches includes studies of novel therapeutic approaches, such as immunomodulation, gene therapy, nutrition, and others. The mission of the Journal is to foster and promote multidisciplinary studies, both experimental and clinical in nature, that critically examine the etiology, mechanisms and novel therapeutics of shock-related pathophysiological conditions. Its purpose is to excel as a vehicle for timely publication in the areas of basic and clinical studies of shock, trauma, sepsis, inflammation, ischemia, and related pathobiological states, with particular emphasis on the biologic mechanisms that determine the response to such injury. Making such information available will ultimately facilitate improved care of the traumatized or septic individual.