The Identification of a New Gene KRTAP 6-3 in Capra hircus and Its Potential for the Diameter Improvement of Cashmere Fibers.

IF 2.8 3区 生物学 Q2 GENETICS & HEREDITY
Genes Pub Date : 2025-06-19 DOI:10.3390/genes16060721
Jian Cao, Zhanzhao Chen, Jianmin Zhang, Liang Cao, Shaobin Li
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引用次数: 0

Abstract

Background: Cashmere is one of the important economic products of goats, and the KRTAP gene family, as an important family of regulatory genes in the growth process of cashmere fiber, largely affects the quality of cashmere.

Methods: In this study, the KRTAP6-3 gene was identified and located on goat chromosome 1 using a goat genome homology search combined with a phylogenetic tree approach. The Longdong cashmere goat KRTAP6-3 gene variation and its effect on cashmere quality were explored by using the polymerase chain reaction single-stranded conformation polymorphism (PCR-SSCP) technique, in situ hybridization, and the allele presence/absence model.

Results: The results identified a total of six SNPs in KRTAP6-3, three of which were located in the coding region and two of which were synonymous mutations, in addition to 45- bp deletion sequences detected in alleles C and F. Moreover, the KRTAP6-3 mRNA showed a strong expression signal in the cortical layer of the primary and secondary follicles in the inner root sheaths, as well as in the cells of the hair papillae and the matrices during the anagen phase, and signaling at the sites described above is attenuated during the telogen phase. The presence of allele C was associated with increased MFD (mean fiber diameter) (p < 0.01). The MFD of goats with allele C genotype (genotype AC) was significantly higher (p < 0.05) than that of goats without allele C genotype (genotypes AA and AB).

Conclusions: This indicates that genetic variation in the KRTAP6-3 gene in goats is significantly associated with cashmere traits and can serve as a candidate gene for molecular markers of cashmere traits.

山羊绒新基因KRTAP 6-3的鉴定及其在羊绒纤维增径中的应用前景。
背景:羊绒是山羊重要的经济产品之一,KRTAP基因家族作为羊绒纤维生长过程中重要的调控基因家族,在很大程度上影响着羊绒的品质。方法:采用山羊基因组同源性检索结合系统发育树方法,在山羊1号染色体上鉴定并定位KRTAP6-3基因。采用聚合酶链反应单链构象多态性(PCR-SSCP)技术、原位杂交和等位基因存在/缺失模型,对陇东绒山羊KRTAP6-3基因变异及其对羊绒品质的影响进行了研究。结果:结果发现KRTAP6-3共有6个snp,其中3个位于编码区,2个为同音突变,另外在等位基因C和f中检测到45- bp的缺失序列。KRTAP6-3 mRNA在毛根内鞘的初级毛囊和次级毛囊皮质层以及毛乳头细胞和毛囊基质中表现出较强的表达信号。上述位点的信号在休止期减弱。等位基因C的存在与MFD(平均纤维直径)增加相关(p < 0.01)。携带等位基因C基因型(AC型)山羊的MFD显著高于不携带等位基因C基因型(AA和AB型)山羊(p < 0.05)。结论:山羊KRTAP6-3基因的遗传变异与羊绒性状显著相关,可作为羊绒性状分子标记的候选基因。
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来源期刊
Genes
Genes GENETICS & HEREDITY-
CiteScore
5.20
自引率
5.70%
发文量
1975
审稿时长
22.94 days
期刊介绍: Genes (ISSN 2073-4425) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to genes, genetics and genomics. It publishes reviews, research articles, communications and technical notes. There is no restriction on the length of the papers and we encourage scientists to publish their results in as much detail as possible.
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