Variability of carotenoid synthesis and degradation genes in Russian durum wheat cultivars.

IF 0.9 Q3 AGRICULTURE, MULTIDISCIPLINARY
A A Trifonova, K V Boris, L V Dedova, P N Malchikov, A M Kudryavtsev
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引用次数: 0

Abstract

Yellow index is an important quality parameter of durum wheat cultivars, associated with carotenoid pigment content in grain and the level of carotenoid degradation during processing, and determining the yellow color of products made from durum wheat. Molecular markers of genes that influence carotenoid content can be used for fast identification of valuable genotypes and development of new high-quality durum wheat cultivars. The aim of the study was to investigate the domestic durum wheat gene pool using molecular markers of the yellow pigment synthesis (Psy-A1) and degradation (Lpx-B1) genes. Using two markers of the phytoene synthase Psy- A1 gene (PSY1-A1_STS and YP7A-2) and three markers of the lipoxygenase Lpx-B1 locus (Lpx-B1.1a/1b, Lpx- B1.1c and Lpx- B1.2/1.3), 54 durum wheat cultivars were studied for the first time. For 38 cultivars, yellow pigment content in grain was also assessed. The detected allelic variation of the phytoene synthase Psy-A1 and lipoxygenase Lpx-B1 genes was rather low. The most common Psy-A1 alleles among the studied cultivars were Psy-A1l for the PSY1- A1_STS marker and Psy-A1d for the YP7A-2 marker, identified in 51 cultivars and associated with high carotenoid content. According to the markers of the Lpx-B1 locus, haplotype II, associated with medium lipoxygenase activity, identified in 43 cultivars, was predominant. Haplotype III, associated with low enzyme activity, was identified in only three winter durum wheat cultivars (Donchanka, Gelios and Leucurum 21). Despite the predominance of allelic variants associated with increased carotenoid content and moderate lipoxygenase activity, the studied cultivars had different levels of yellow pigment content in grain, from low to high.

俄罗斯硬粒小麦品种类胡萝卜素合成和降解基因的变异。
黄色指数是硬粒小麦品种的重要品质参数,与籽粒中类胡萝卜素的含量和加工过程中类胡萝卜素的降解水平有关,决定了硬粒小麦制品的黄色。影响类胡萝卜素含量的基因分子标记可用于快速鉴定有价值的基因型和培育优质硬粒小麦新品种。利用黄色色素合成基因(Psy-A1)和降解基因(Lpx-B1)的分子标记对国内硬粒小麦基因库进行了研究。利用植物烯合成酶Psy- A1基因的2个标记(PSY1-A1_STS和YP7A-2)和脂氧合酶Lpx- b1位点的3个标记(Lpx- b1.1 a/1b、Lpx- B1.1c和Lpx- B1.2/1.3),首次对54个硬粒小麦品种进行了研究。对38个品种的籽粒中黄色色素含量进行了测定。检测到的植物烯合成酶ppx - a1和脂氧合酶Lpx-B1等位基因变异较低。在所研究的51个品种中,最常见的Psy-A1等位基因是PSY1- a1 - sts标记的Psy-A1和YP7A-2标记的Psy-A1,这些等位基因与高类胡萝卜素含量相关。根据Lpx-B1位点的标记,在43个品种中鉴定出与中等脂氧合酶活性相关的II型单倍型占优势。单倍型III与低酶活性相关,仅在冬硬小麦品种(Donchanka、Gelios和Leucurum 21)中被鉴定出来。尽管与类胡萝卜素含量增加和脂氧合酶活性中等相关的等位基因变异占优势,但所研究的品种籽粒中黄色色素含量由低到高存在不同水平。
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来源期刊
Vavilovskii Zhurnal Genetiki i Selektsii
Vavilovskii Zhurnal Genetiki i Selektsii AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
1.90
自引率
0.00%
发文量
119
审稿时长
8 weeks
期刊介绍: The "Vavilov Journal of genetics and breeding" publishes original research and review articles in all key areas of modern plant, animal and human genetics, genomics, bioinformatics and biotechnology. One of the main objectives of the journal is integration of theoretical and applied research in the field of genetics. Special attention is paid to the most topical areas in modern genetics dealing with global concerns such as food security and human health.
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