Protease-Catalyzed Synthesis of Water-Soluble Oligohistidine.

Abstract

This article presents a "green" approach to water-soluble oligohistidine peptides through protease-catalyzed peptide synthesis. Synthesis was catalyzed by papain and α-chymotrypsin, and reactions were monitored in-situ by real-time 1-hour NMR spectroscopy. Pyrosetta computational modeling was used to calculate the energy required for amidation versus hydrolysis with papain and α-chymotrypsin.