Self-Assembly of 2-Hydroxyethyl-1H-imidazolium-Based Surface Active Ionic Liquids and Utilization of Their Aqueous Solution in Superactivity of Cytochrome-c.

IF 2.8 2区 化学 Q3 CHEMISTRY, PHYSICAL
Manpreet Singh, Rajwinder Kaur, Sugam Kumar, Vinod Kumar Aswal, Gurbir Singh, Tejwant Singh Kang
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引用次数: 0

Abstract

Imidazolium-based surface active ionic liquids (SAILs) appended with a hydroxyethyl moiety at a cationic headgroup at a position opposite to an alkyl chain ([C12ImOH][Br] and [C16ImOH][Br]) and alkyl chain functionalized with amide ([C12AImOH][Br]) and ester ([C12EImOH][Br]) groups have been synthesized. Different techniques, i.e., surface tension, ionic conductance, fluorescence, dynamic light scattering, small angle neutron scattering, and isothermal titration calorimetry, have been exploited for establishing their micellization behavior, followed by the utilization of aqueous SAILs to offer enhanced enzymatic activity to cytochrome-c (Cyt-c). The hydrophobic hydration of the hydroxyethyl group retards the micellization in bulk but disturbs the water structure at and beneath the air-solution interface, resulting in better surface-active behavior as compared to their nonhydroxyethyl functionalized counterparts. The variation of characteristic micellar properties, i.e., cmc, counterion binding (β), aggregation number (Nagg), size, compactness, and thermodynamic parameters of micellization, is supported by the increasing hydrophobicity of the cationic SAIL along with contrastingly different H-bonding and stiffness of ester or amide moiety present near the cationic headgroup. The aqueous solutions of SAILs (below cmc) favored the enzyme activity governed predominantly by hydrophobic as well as polar interactions between SAILs and Cyt-c corroborated by molecular docking and circular dichroism (CD) spectroscopy investigations. Cyt-c shows a retarded activity in aqueous micelles of SAILs (above cmc) with the exception of [C12AImOH][Br], where enzyme activity increased further to ∼2.1-fold compared to that observed in buffer. It is anticipated that the current results would pave a new way to synthesize SAILs not only to be used for enzyme activation and prolonged storage in aqueous medium but also for other biological applications.

2-羟乙基- 1h -咪唑基表面活性离子液体的自组装及其水溶液在细胞色素-c超活性中的应用。
合成了在与烷基链([C12ImOH][Br]和[C16ImOH][Br])相反的阳离子头基上附羟乙基基团的咪唑基表面活性离子液体(SAILs),以及与酰胺([C12AImOH][Br])和酯([C12EImOH][Br])官能化的烷基链。不同的技术,如表面张力、离子电导、荧光、动态光散射、小角中子散射和等温滴定量热法,已经被用来建立它们的胶束行为,然后利用水性帆来增强细胞色素c (Cyt-c)的酶活性。羟乙基的疏水水合作用阻碍了整体胶束化,但扰乱了空气-溶液界面及其下方的水结构,与非羟乙基功能化的对应物相比,具有更好的表面活性行为。阳离子SAIL的疏水性增强以及阳离子头基附近酯或酰胺部分的h键和刚度的差异支持了其胶束特性(cmc、反离子结合(β)、聚集数(Nagg)、尺寸、致密性和胶束化热力学参数)的变化。通过分子交会和圆二色性(CD)光谱研究证实,sail的水溶液(低于cmc)有利于主要受疏水和极性相互作用支配的酶活性。除了[C12AImOH][Br]外,Cyt-c在sail(高于cmc)的水性胶束中表现出活性迟缓,其酶活性比在缓冲液中观察到的活性进一步增加到约2.1倍。预计目前的研究结果将为合成用于酶活化和在水介质中长期储存的帆状膜铺平新的道路,并将用于其他生物应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
5.80
自引率
9.10%
发文量
965
审稿时长
1.6 months
期刊介绍: An essential criterion for acceptance of research articles in the journal is that they provide new physical insight. Please refer to the New Physical Insights virtual issue on what constitutes new physical insight. Manuscripts that are essentially reporting data or applications of data are, in general, not suitable for publication in JPC B.
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