Crystal structure of the sucrose phosphorylase from Alteromonas mediterranea shows a loop transition in the active site

IF 1.1 4区 生物学 Q4 BIOCHEMICAL RESEARCH METHODS
Folmer Fredslund, Marine Goux, Bernard Offmann, Marie Demonceaux, Corinne André-Miral, Ditte Welner, David Teze
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引用次数: 0

Abstract

Sucrose phosphorylases are essential enzymes regulating sucrose metabolism, and it has been shown that a loop rearrangement is essential to their catalytic cycle. Crystal structures of only six sucrose phosphorylase enzymes are available. Here, we present the crystal structure of a sucrose phosphorylase from a proteobacterium, Alteromonas mediterranea, at 2.15 Å resolution. The available sucrose phosphorylase structures have shown that an important conformational change occurs during the catalytic cycle or upon mutagenesis. Interestingly, our data present clear indications of the two major conformations in the same crystal.

Abstract Image

地中海交替单胞菌蔗糖磷酸化酶的晶体结构在活性位点呈环状转变。
蔗糖磷酸化酶是调节蔗糖代谢的重要酶,研究表明,环重排是其催化循环的必要条件。只有六种蔗糖磷酸化酶的晶体结构是可用的。在这里,我们以2.15 Å的分辨率展示了一种变形菌,地中海交替单胞菌的蔗糖磷酸化酶的晶体结构。现有的蔗糖磷酸化酶结构表明,在催化循环或诱变过程中发生了重要的构象变化。有趣的是,我们的数据清楚地显示了同一晶体中的两种主要构象。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Acta crystallographica. Section F, Structural biology communications
Acta crystallographica. Section F, Structural biology communications BIOCHEMICAL RESEARCH METHODSBIOCHEMISTRY &-BIOCHEMISTRY & MOLECULAR BIOLOGY
CiteScore
1.90
自引率
0.00%
发文量
95
期刊介绍: Acta Crystallographica Section F is a rapid structural biology communications journal. Articles on any aspect of structural biology, including structures determined using high-throughput methods or from iterative studies such as those used in the pharmaceutical industry, are welcomed by the journal. The journal offers the option of open access, and all communications benefit from unlimited free use of colour illustrations and no page charges. Authors are encouraged to submit multimedia content for publication with their articles. Acta Cryst. F has a dedicated online tool called publBio that is designed to make the preparation and submission of articles easier for authors.
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