Registration of six Oryza rufipogon species complex (ORSC) chromosome segment substitution line (CSSL) libraries in elite Indica and Japonica rice backgrounds

IF 0.6 4区农林科学 Q4 AGRONOMY

Journal of Plant Registrations Pub Date : 2025-06-22 DOI:10.1002/plr2.70002

Georgia C. Eizenga, Namrata Singh, M Liakat Ali, Ehsan Shakiba, Kazi M. Akther, Sandra E. Harrington, Susan R. McCouch

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引用次数: 0

Abstract

The two varietal groups of cultivated rice (Oryza sativa) are Indica and Japonica. Ancestral species O. rufipogon and O. nivara, collectively identified as the Oryza rufipogon species complex (ORSC), represent an underutilized resource for rice improvement. To make the ORSC genepool more accessible for breeding, three genotypically and phenotypically diverse ORSC accessions were selected as donors to develop chromosome segment substitution line (CSSL) libraries from crosses with IR64, an indica long grain grown in South and Southeast Asia, and Cybonnet, a U.S. tropical japonica long grain. The Cybonnet (Cyb) x OrA (O. rufipogon, NIAS W1944) CSSL Library (Reg. no. MP-11, NSL 551190 MAP) and IR64 x OrA (O. rufipogon, NIAS W1944) CSSL Library (Reg. no. MP-14, NSL 551377 MAP) used an ORSC donor from China, Cybonnet (Cyb) x OrB (O. nivara, IRGC106148) CSSL Library (Reg. no. MP-12, NSL 551297 MAP) and IR64 x OrB (O. nivara, IRGC106148) CSSL Library (Reg. no. MP-15, NSL 551378 MAP) an ORSC donor from Laos, and Cybonnet (Cyb) x OrC (O. rufipogon, IRGC105567) CSSL Library (Reg. no. MP-13, NSL 551376 MAP) and IR64 x OrC (O. rufipogon, IRGC105567) CSSL Library (Reg. no. MP-16, NSL 551379 MAP) an ORSC donor from Indonesia. The libraries were genotyped with an Infinium 7K array for rice. Previously, Cybonnet and 212 of the 216 Cybonnet CSSLs from the three libraries were evaluated in replicated field studies for six agronomic, six panicle architecture, and eight seed traits. Similarly, IR64 and 218 IR64 CSSLs from three libraries were grown in the greenhouse and characterized for six agronomic traits, panicle type and length, and four seed traits. Awn presence and culm color were noted for all CSSLs with three underlying genes identified. Of the 28 candidate genes identified by substitution mapping in the Cybonnet libraries, 11 were validated for the same traits phenotyped in the IR64 libraries. Phenotypes of CSSLs with ORSC introgressions that included the LK3, Waxy, ALK, and Pita genes validated the ORSC allele for these genes. These six CSSL libraries are a unique resource for researchers to discover novel alleles in the ORSC donors for rice improvement.

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6个水稻物种复合体（ORSC）染色体片段代换系（CSSL）文库在籼稻和粳稻优良背景下的注册

栽培水稻（Oryza sativa）的两个品种群是籼稻和粳稻。祖先种O. rufipogon和O. nivara统称为Oryza rufipogon物种复合体（ORSC），是水稻改良中未充分利用的资源。为了使ORSC基因库更容易用于育种，选择了3个基因型和表型多样化的ORSC材料作为供体，与生长在南亚和东南亚的籼稻长粒IR64和美国热带粳稻长粒Cybonnet杂交，建立染色体片段代换系（CSSL）文库。Cybonnet (Cyb) x OrA (O. rufipogon, NIAS W1944) CSSL文库（Reg.）否。MP-11, NSL 551190 MAP)和IR64 x OrA (O. rufipogon, NIAS W1944) CSSL库（Reg.）。否。MP-14, NSL 551377 MAP)使用来自中国的ORSC供体Cybonnet (Cyb) x OrB (O. nivara, IRGC106148) CSSL Library （Reg.）。否。MP-12, NSL 551297 MAP)和IR64 x OrB (O. nivara, IRGC106148) CSSL库(Reg。否。MP-15, NSL 551378 MAP)来自老挝的ORSC供体，Cybonnet (Cyb) x OrC (O. rufipogon, IRGC105567) CSSL库（注册）；否。MP-13, NSL 551376 MAP)和IR64 x OrC (O. rufipogon, IRGC105567) CSSL库(Reg。否。MP-16, NSL 551379 MAP)来自印度尼西亚的ORSC捐赠者。文库用Infinium 7K水稻阵列进行基因分型。此前，对三个文库中216份Cybonnet CSSLs中的212份Cybonnet CSSLs进行了6项农艺性状、6项穗型性状和8项种子性状的重复田间评价。同样，从3个文库中获得的IR64和218个IR64 CSSLs在温室中生长，并对其进行了6个农艺性状（穗型和穗长）和4个种子性状的鉴定。所有的CSSLs都有芒的存在和秆的颜色，并鉴定了三个潜在基因。在Cybonnet文库中通过替代定位鉴定的28个候选基因中，有11个在IR64文库中具有相同的表型。包括LK3、Waxy、ALK和Pita基因在内的ORSC基因渗入的cssl表型证实了这些基因的ORSC等位基因。这6个基因库为研究人员在ORSC供体中发现新的等位基因提供了独特的资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Plant Registrations 农林科学-农艺学

CiteScore

1.60

自引率

37.50%

发文量

审稿时长

2 months

期刊介绍： The Journal of Plant Registrations is an official publication of the Crop Science Society of America and the premier international venue for plant breeders, geneticists, and genome biologists to publish research describing new and novel plant cultivars, germplasms, parental lines, genetic stocks, and genomic mapping populations. In addition to biomedical, nutritional, and agricultural scientists, the intended audience includes policy makers, humanitarian organizations, and all facets of food, feed, fiber, bioenergy, and shelter industries. The scope of articles includes (1) cultivar, germplasm, parental line, genetic stock, and mapping population registration manuscripts, (2) short manuscripts characterizing accessions held within Plant Germplasm Collection Systems, and (3) descriptions of plant genetic materials that have made a major impact on agricultural security. Registration of plant genetic resources, item (1) above, requires deposit of plant genetic material into the USDA ARS National Plant Germplasm System prior to publication.