TKT regulates the pentose phosphate pathway via RBKS to promote epithelial-mesenchymal transition during AML progression.

IF 2.9 4区 生物学 Q2 BIOPHYSICS
Feifan Li, Jiaqi Liu, Yinghua Geng, Lin Liu, Jun Li, Lianfang Pu, Zhongli Hu, Yanli Yang
{"title":"TKT regulates the pentose phosphate pathway via RBKS to promote epithelial-mesenchymal transition during AML progression.","authors":"Feifan Li, Jiaqi Liu, Yinghua Geng, Lin Liu, Jun Li, Lianfang Pu, Zhongli Hu, Yanli Yang","doi":"10.1007/s10863-025-10064-z","DOIUrl":null,"url":null,"abstract":"<p><p>Acute myeloid leukemia is a life-threaten disease. Researches have indicated that increased expression of TKT was closely related to the progression of malignant tumors. However, the mechanism of TKT in the pathogenesis of AML need to be further elucidated. Here, we showed that the expression levels of TKT was increased in AML patients and AML cells. TKT overexpression in AML cells significantly promoted the proliferation, migration and invasion of cells while TKT knockdown had opposite effects. Mechanistically. We proved that TKT was located on up-stream of RBKS and TKT promoted the growth of AML cells through RBKS. In addition, our data indicated that TKT regulates the pentose phosphate pathway via RBKS. Notably, we demonstrated that the pentose phosphate pathway is crucial for EMT program in AML cells. Taken together, this study identified the molecular mechanism by which TKT promotes AML progression, namely, TKT promotes EMT by regulating the pentose phosphate pathway through RBKS. Our results suggest that TKT maybe a novel therapeutic target for AML treatment.</p>","PeriodicalId":15080,"journal":{"name":"Journal of Bioenergetics and Biomembranes","volume":" ","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Bioenergetics and Biomembranes","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10863-025-10064-z","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOPHYSICS","Score":null,"Total":0}
引用次数: 0

Abstract

Acute myeloid leukemia is a life-threaten disease. Researches have indicated that increased expression of TKT was closely related to the progression of malignant tumors. However, the mechanism of TKT in the pathogenesis of AML need to be further elucidated. Here, we showed that the expression levels of TKT was increased in AML patients and AML cells. TKT overexpression in AML cells significantly promoted the proliferation, migration and invasion of cells while TKT knockdown had opposite effects. Mechanistically. We proved that TKT was located on up-stream of RBKS and TKT promoted the growth of AML cells through RBKS. In addition, our data indicated that TKT regulates the pentose phosphate pathway via RBKS. Notably, we demonstrated that the pentose phosphate pathway is crucial for EMT program in AML cells. Taken together, this study identified the molecular mechanism by which TKT promotes AML progression, namely, TKT promotes EMT by regulating the pentose phosphate pathway through RBKS. Our results suggest that TKT maybe a novel therapeutic target for AML treatment.

在AML进展过程中,TKT通过RBKS调控戊糖磷酸途径,促进上皮-间质转化。
急性髓性白血病是一种危及生命的疾病。研究表明,TKT表达的增加与恶性肿瘤的发展密切相关。然而,TKT在AML发病中的作用机制有待进一步阐明。在这里,我们发现TKT在AML患者和AML细胞中的表达水平升高。AML细胞中TKT过表达可显著促进细胞的增殖、迁移和侵袭,而TKT敲低则相反。从力学上看。我们证明TKT位于RBKS的上游,TKT通过RBKS促进AML细胞的生长。此外,我们的数据表明,TKT通过RBKS调节戊糖磷酸途径。值得注意的是,我们证明了戊糖磷酸途径对AML细胞中的EMT程序至关重要。综上所述,本研究确定了TKT促进AML进展的分子机制,即TKT通过RBKS调节戊糖磷酸途径促进EMT。我们的研究结果表明,TKT可能是AML治疗的一个新的治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
6.00
自引率
0.00%
发文量
22
审稿时长
6-12 weeks
期刊介绍: The Journal of Bioenergetics and Biomembranes is an international journal devoted to the publication of original research that contributes to fundamental knowledge in the areas of bioenergetics, biomembranes, and transport, including oxidative phosphorylation, photosynthesis, muscle contraction, as well as cellular and systemic metabolism. The timely research in this international journal benefits biophysicists, membrane biologists, cell biologists, biochemists, molecular biologists, physiologists, endocrinologists, and bio-organic chemists.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信