Cotyledon opening during seedling deetiolation is determined by ABA-mediated splicing regulation.

IF 6.5 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

EMBO Reports Pub Date : 2025-06-18 DOI:10.1038/s44319-025-00495-5

Guiomar Martín, Ana Confraria, Irene Zapata, Alvaro Santiago Larran, Julia Irene Qüesta, Paula Duque

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Abstract

During seedling deetiolation, plants adjust their development to expose photosynthetic tissues to sunlight, enabling the transition from heterotrophic to autotrophic growth. While various plant hormones are known to influence this process, the role of abscisic acid (ABA) remains unclear. Here, we reveal that ABA plays a major role in controlling the dynamics of cotyledon aperture during seedling deetiolation. In the dark, ABA accumulates in the cotyledons to effectively repress their opening. However, light exposure reverses this effect, allowing the cotyledons to open. Our findings indicate that ABA-mediated regulation of cotyledon dynamics is accompanied by genome-wide rearrangements in both transcriptional and splicing patterns. We demonstrate that ABA-dependent adjustments of cotyledon and splicing dynamics in response to light depend on the positive role of two splicing factors, RS40 and RS41. Moreover, we identify transcriptional and posttranscriptional mechanisms that control the activity of these proteins. Altogether, this work sheds light on the interplay between light and ABA, highlighting cotyledon opening as a new developmental outcome, and identifying alternative splicing as the underlying layer of gene regulation.

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幼苗脱落过程中子叶的开放是由aba介导的剪接调控决定的。

在幼苗脱腐过程中，植物调整其发育，使光合组织暴露在阳光下，使其从异养生长过渡到自养生长。虽然已知多种植物激素会影响这一过程，但脱落酸（ABA）的作用尚不清楚。本研究发现，ABA在幼苗脱叶过程中对子叶孔径的动态调控起着重要作用。在黑暗中，ABA在子叶中积累，有效地抑制子叶的开放。然而，光照会逆转这种效果，让子叶打开。我们的研究结果表明，aba介导的子叶动力学调节伴随着转录和剪接模式的全基因组重排。我们证明了aba对子叶和剪接动态响应的调节依赖于两个剪接因子RS40和RS41的积极作用。此外，我们确定了控制这些蛋白质活性的转录和转录后机制。总之，这项工作揭示了光与ABA之间的相互作用，强调子叶开放是一种新的发育结果，并确定了选择性剪接是基因调控的底层。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

EMBO Reports 生物-生化与分子生物学

CiteScore

11.20

自引率

1.30%

发文量

267

审稿时长

1 months

期刊介绍： EMBO Reports is a scientific journal that specializes in publishing research articles in the fields of molecular biology, cell biology, and developmental biology. The journal is known for its commitment to publishing high-quality, impactful research that provides novel physiological and functional insights. These insights are expected to be supported by robust evidence, with independent lines of inquiry validating the findings. The journal's scope includes both long and short-format papers, catering to different types of research contributions. It values studies that: Communicate major findings: Articles that report significant discoveries or advancements in the understanding of biological processes at the molecular, cellular, and developmental levels. Confirm important findings: Research that validates or supports existing knowledge in the field, reinforcing the reliability of previous studies. Refute prominent claims: Studies that challenge or disprove widely accepted ideas or hypotheses in the biosciences, contributing to the correction and evolution of scientific understanding. Present null data: Papers that report negative results or findings that do not support a particular hypothesis, which are crucial for the scientific process as they help to refine or redirect research efforts. EMBO Reports is dedicated to maintaining high standards of scientific rigor and integrity, ensuring that the research it publishes contributes meaningfully to the advancement of knowledge in the life sciences. By covering a broad spectrum of topics and encouraging the publication of both positive and negative results, the journal plays a vital role in promoting a comprehensive and balanced view of scientific inquiry.