Combined X-ray and neutron tomography for simultaneous assessment of in silico mechanical response and in vitro biological behavior of graded scaffolds: a preliminary study

IF 6.8 3区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Journal of Science: Advanced Materials and Devices Pub Date : 2025-06-14 DOI:10.1016/j.jsamd.2025.100935

Maria Laura Gatto , Fabrizio Fiori , Alessandro Tengattini , Lukas Helfen , Marcello Cabibbo , Mattia Utzeri , Michele Furlani , Giorgia Cerqueni , Daniela Lamanna , Monica Mattioli-Belmonte , Paolo Mengucci

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引用次数: 0

Abstract

This study presents a pioneering approach combining X-ray Computed Tomography (XCT) and Neutron Computed Tomography (NCT) to simultaneously assess the biomechanical and biological behavior of bone scaffolds fabricated by additive manufacturing. XCT was employed to model post-fabrication scaffold geometry, enabling precise finite element (FE) predictions on how each defect type, such as strut densification, lack of fusion and porosity, impacts the overall mechanical performance of the scaffold. Defect-inclusive models were successfully validated against experimental data. NCT was instead concomitantly used to assess short-term changes in the behavior of MG-63 human osteoblast-like cells under different culture conditions. Quantitative analysis of NCT scans of our samples showed that neutrons, thanks to their sensitivity to organic material, are able to discriminate between different compositions of the organic material itself. This research not only provides a comprehensive framework for preclinical characterization of scaffolds, but also paves the way for more efficient evaluation of implantable devices, ultimately reducing the reliance on animal models in biomaterials research.

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联合x射线和中子层析成像同时评估分级支架的硅力学响应和体外生物学行为：初步研究

本研究提出了一种开创性的方法，结合x射线计算机断层扫描（XCT）和中子计算机断层扫描（NCT），同时评估增材制造制造的骨支架的生物力学和生物学行为。XCT用于模拟制造后支架的几何形状，从而实现对每种缺陷类型（如支撑致密化、缺乏融合和孔隙度）如何影响支架整体力学性能的精确有限元（FE）预测。根据实验数据成功验证了包含缺陷的模型。相反，NCT同时用于评估MG-63人成骨样细胞在不同培养条件下行为的短期变化。对样品的NCT扫描的定量分析表明，由于中子对有机材料的敏感性，它们能够区分有机材料本身的不同成分。本研究不仅为支架的临床前表征提供了一个全面的框架，而且为更有效地评估可植入装置铺平了道路，最终减少了生物材料研究对动物模型的依赖。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Science: Advanced Materials and Devices Materials Science-Electronic, Optical and Magnetic Materials

CiteScore

11.90

自引率

2.50%

发文量

审稿时长

47 days

期刊介绍： In 1985, the Journal of Science was founded as a platform for publishing national and international research papers across various disciplines, including natural sciences, technology, social sciences, and humanities. Over the years, the journal has experienced remarkable growth in terms of quality, size, and scope. Today, it encompasses a diverse range of publications dedicated to academic research. Considering the rapid expansion of materials science, we are pleased to introduce the Journal of Science: Advanced Materials and Devices. This new addition to our journal series offers researchers an exciting opportunity to publish their work on all aspects of materials science and technology within the esteemed Journal of Science. With this development, we aim to revolutionize the way research in materials science is expressed and organized, further strengthening our commitment to promoting outstanding research across various scientific and technological fields.