Overexpression of LINC00880 promotes colorectal cancer growth.

IF 1.5 4区医学 Q4 ONCOLOGY

Translational cancer research Pub Date : 2025-05-30 Epub Date: 2025-05-23 DOI:10.21037/tcr-2025-54

Ge Gao, Peiwen Xu, Chunyu Yang, Mengyuan Qian, Qingwen Wang, Surui Yao, Yuan Yin, Zhaohui Huang, Zehua Bian

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引用次数: 0

Abstract

Background: Research has revealed that long non-coding RNAs (lncRNAs) are intimately associated with the occurrence, development, and metastasis of tumors through their regulation of gene expression. The lncRNA LINC00880 is important for colorectal cancer (CRC) occurrence and development. Our research aimed to explore the roles of LINC00880 in CRC progression.

Methods: The expression of LINC00880 in CRC cells and tissues was first measured using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and the prognosis of CRC patients was then investigated using the Kaplan-Meier method. The impacts of LINC00880 on CRC growth were evaluated by a series of in vitro and in vivo assays. Mechanistically, RNA sequencing (RNA-seq) technology and transcriptome analysis experiments were employed to validate the impact of LINC00880 on cell cycle pathway.

Results: In this study, we have demonstrated, for the first time, that LINC00880 is significantly overexpressed in CRC and is associated with poor patient survival. Functional assays indicated that LINC00880 promotes the growth of CRC cells both in vitro and in vivo. Furthermore, RNA-seq has revealed the impact of LINC00880 on the cell cycle and DNA replication pathways, and identified MCM3 as a potential downstream target of LINC00880.

Conclusions: Our findings indicate that LINC00880 is upregulated in CRC and promotes tumor growth.

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过表达LINC00880促进结直肠癌的生长。

背景：研究表明，长链非编码rna （long non-coding RNAs, lncRNAs）通过调控基因表达与肿瘤的发生、发展和转移密切相关。lncRNA LINC00880在结直肠癌（CRC）的发生和发展中具有重要意义。我们的研究旨在探讨LINC00880在结直肠癌进展中的作用。方法：首先采用定量逆转录聚合酶链式反应（qRT-PCR）检测LINC00880在结直肠癌细胞和组织中的表达，然后采用Kaplan-Meier法研究结直肠癌患者的预后。通过一系列体外和体内实验评估LINC00880对结直肠癌生长的影响。机制上，采用RNA测序（RNA-seq）技术和转录组分析实验验证LINC00880对细胞周期通路的影响。结果：在这项研究中，我们首次证明了LINC00880在CRC中显著过表达，并与患者生存率低相关。功能实验表明，LINC00880在体外和体内均能促进结直肠癌细胞的生长。此外，RNA-seq揭示了LINC00880对细胞周期和DNA复制途径的影响，并确定MCM3是LINC00880的潜在下游靶点。结论：我们的研究结果表明，LINC00880在结直肠癌中表达上调，促进肿瘤生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational cancer research ONCOLOGY-

CiteScore

2.10

自引率

0.00%

发文量

252

期刊介绍： Translational Cancer Research (Transl Cancer Res TCR; Print ISSN: 2218-676X; Online ISSN 2219-6803; http://tcr.amegroups.com/) is an Open Access, peer-reviewed journal, indexed in Science Citation Index Expanded (SCIE). TCR publishes laboratory studies of novel therapeutic interventions as well as clinical trials which evaluate new treatment paradigms for cancer; results of novel research investigations which bridge the laboratory and clinical settings including risk assessment, cellular and molecular characterization, prevention, detection, diagnosis and treatment of human cancers with the overall goal of improving the clinical care of cancer patients. The focus of TCR is original, peer-reviewed, science-based research that successfully advances clinical medicine toward the goal of improving patients'' quality of life. The editors and an international advisory group of scientists and clinician-scientists as well as other experts will hold TCR articles to the high-quality standards. We accept Original Articles as well as Review Articles, Editorials and Brief Articles.