{"title":"State of the art indicators for imaging purinergic dynamics in vitro and in vivo.","authors":"Yumo Li, Liwan Zhang, Bohan Li, Yulong Li, Zhaofa Wu","doi":"10.1007/s11302-025-10095-6","DOIUrl":null,"url":null,"abstract":"<p><p>Purinergic neurotransmission, a dynamic signaling system using adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine (ADO), uridine diphosphate (UDP), and others, plays a crucial role in brain function. Purinergic signaling is involved in regulating synaptic communication to influence sleep and neuroprotection; malfunction of purinergic signaling contributes to various neurological disorders like pain, epilepsy, and depression. Effective detection methods are crucial for a comprehensive understanding of the multifaceted roles of purinergic signaling in the brain. This review sheds light on advancements in fluorescent indicators, a powerful toolkit for visualizing purinergic activities in living animals. We explore the diverse applications of these indicators in studying purinergic transmission both in health and in diseases. Despite their current strengths, we emphasize the need for continuous development of fluorescent indicators to achieve an even more comprehensive, specific, and quantitative detection of purinergic signaling.</p>","PeriodicalId":20952,"journal":{"name":"Purinergic Signalling","volume":" ","pages":""},"PeriodicalIF":3.0000,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Purinergic Signalling","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s11302-025-10095-6","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NEUROSCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Purinergic neurotransmission, a dynamic signaling system using adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine (ADO), uridine diphosphate (UDP), and others, plays a crucial role in brain function. Purinergic signaling is involved in regulating synaptic communication to influence sleep and neuroprotection; malfunction of purinergic signaling contributes to various neurological disorders like pain, epilepsy, and depression. Effective detection methods are crucial for a comprehensive understanding of the multifaceted roles of purinergic signaling in the brain. This review sheds light on advancements in fluorescent indicators, a powerful toolkit for visualizing purinergic activities in living animals. We explore the diverse applications of these indicators in studying purinergic transmission both in health and in diseases. Despite their current strengths, we emphasize the need for continuous development of fluorescent indicators to achieve an even more comprehensive, specific, and quantitative detection of purinergic signaling.
期刊介绍：
Nucleotides and nucleosides are primitive biological molecules that were utilized early in evolution both as intracellular energy sources and as extracellular signalling molecules. ATP was first identified as a neurotransmitter and later as a co-transmitter with all the established neurotransmitters in both peripheral and central nervous systems. Four subtypes of P1 (adenosine) receptors, 7 subtypes of P2X ion channel receptors and 8 subtypes of P2Y G protein-coupled receptors have currently been identified. Since P2 receptors were first cloned in the early 1990’s, there is clear evidence for the widespread distribution of both P1 and P2 receptor subtypes in neuronal and non-neuronal cells, including glial, immune, bone, muscle, endothelial, epithelial and endocrine cells.
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