Design, construction and application of an in vitro bionic small intestine absorption model based on dialysis membranes

IF 3.4 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Luping Wang , Zejun Hu , Peng Wu , Zhenkai Liao , Qiyang Ding , Ke Wang , Timothy V. Kirk , Xiao Dong Chen
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Abstract

Ethical, economic, and technical factors limit the use of animals and humans in bioavailability studies involving intestinal absorption. In response, this study introduced an in vitro bionic small intestine absorption model based on a dialysis membrane (BSIA), and systematically examined its performance in mimicking nutrient absorption in the small intestine. It replicated key functions of the human small intestine, including peristalsis and osmosis. Effective permeability coefficients (Peff) for glucose solutions increased from 4.24 ± 0.45 × 10−5 cm/s at 10 mg/mL, to 7.13 ± 0.21 × 10−5 cm/s at 100 mg/mL. No significant differences in Peff were observed for varying membrane cut-off molecular weights. In the first in vitro digestion study of real foods with a dialysis membrane model, milk and rice displayed absorption rates of 74.14 ± 2.46 % for amino acids from milk, and 65.39 ± 2.53 % for glucose from rice, with corresponding Peff values of 4.96 ± 0.25 × 10⁻⁵ cm/s and 7.30 ± 0.36 × 10⁻⁵ cm/s, respectively. These values were comparable with Caco-2 cell monolayer models and excised rat small intestine perfusion studies. They were lower than typical values from human studies, but analysis indicated that transmembrane pressure could be used to increase them. These findings demonstrate that the BSIA effectively simulates nutrient permeation, providing a valuable tool for studying intestinal absorption.
基于透析膜的体外仿生小肠吸收模型的设计、构建及应用
伦理、经济和技术因素限制了在涉及肠道吸收的生物利用度研究中使用动物和人类。为此,本研究建立了一种基于透析膜(BSIA)的体外仿生小肠吸收模型,并对其模拟小肠营养吸收的性能进行了系统研究。它复制了人类小肠的关键功能,包括蠕动和渗透。有效渗透率系数对葡萄糖(Peff)解决方案增加从4.24 ±0.45  × 10−5 cm / s mg / mL, 10点到7.13 ±0.21  × 10−5 cm / s 100 毫克/毫升。不同的膜截止分子量对Peff没有显著的影响。在第一个体外消化与透析膜模型研究真正的食物,牛奶和大米显示吸收率74.14 ±2.46  %从牛奶、氨基酸和65.39 ±2.53  %葡萄糖的大米,用相应的Peff值4.96 ±0.25  ×10 ⁻⁵ 厘米/秒和7.30±0.36  ×10 ⁻⁵cm / s,分别。这些数值与Caco-2细胞单层模型和切除大鼠小肠灌注研究具有可比性。它们低于人体研究的典型值,但分析表明,可以使用跨膜压力来增加它们。这些发现表明BSIA可以有效模拟营养物质的渗透,为研究肠道吸收提供了有价值的工具。
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来源期刊
Food and Bioproducts Processing
Food and Bioproducts Processing 工程技术-工程:化工
CiteScore
9.70
自引率
4.30%
发文量
115
审稿时长
24 days
期刊介绍: Official Journal of the European Federation of Chemical Engineering: Part C FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering. Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing. The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those: • Primarily concerned with food formulation • That use experimental design techniques to obtain response surfaces but gain little insight from them • That are empirical and ignore established mechanistic models, e.g., empirical drying curves • That are primarily concerned about sensory evaluation and colour • Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material, • Containing only chemical analyses of biological materials.
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