Luping Wang , Zejun Hu , Peng Wu , Zhenkai Liao , Qiyang Ding , Ke Wang , Timothy V. Kirk , Xiao Dong Chen
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引用次数: 0
Abstract
Ethical, economic, and technical factors limit the use of animals and humans in bioavailability studies involving intestinal absorption. In response, this study introduced an in vitro bionic small intestine absorption model based on a dialysis membrane (BSIA), and systematically examined its performance in mimicking nutrient absorption in the small intestine. It replicated key functions of the human small intestine, including peristalsis and osmosis. Effective permeability coefficients (Peff) for glucose solutions increased from 4.24 ± 0.45 × 10−5 cm/s at 10 mg/mL, to 7.13 ± 0.21 × 10−5 cm/s at 100 mg/mL. No significant differences in Peff were observed for varying membrane cut-off molecular weights. In the first in vitro digestion study of real foods with a dialysis membrane model, milk and rice displayed absorption rates of 74.14 ± 2.46 % for amino acids from milk, and 65.39 ± 2.53 % for glucose from rice, with corresponding Peff values of 4.96 ± 0.25 × 10⁻⁵ cm/s and 7.30 ± 0.36 × 10⁻⁵ cm/s, respectively. These values were comparable with Caco-2 cell monolayer models and excised rat small intestine perfusion studies. They were lower than typical values from human studies, but analysis indicated that transmembrane pressure could be used to increase them. These findings demonstrate that the BSIA effectively simulates nutrient permeation, providing a valuable tool for studying intestinal absorption.
期刊介绍:
Official Journal of the European Federation of Chemical Engineering:
Part C
FBP aims to be the principal international journal for publication of high quality, original papers in the branches of engineering and science dedicated to the safe processing of biological products. It is the only journal to exploit the synergy between biotechnology, bioprocessing and food engineering.
Papers showing how research results can be used in engineering design, and accounts of experimental or theoretical research work bringing new perspectives to established principles, highlighting unsolved problems or indicating directions for future research, are particularly welcome. Contributions that deal with new developments in equipment or processes and that can be given quantitative expression are encouraged. The journal is especially interested in papers that extend the boundaries of food and bioproducts processing.
The journal has a strong emphasis on the interface between engineering and food or bioproducts. Papers that are not likely to be published are those:
• Primarily concerned with food formulation
• That use experimental design techniques to obtain response surfaces but gain little insight from them
• That are empirical and ignore established mechanistic models, e.g., empirical drying curves
• That are primarily concerned about sensory evaluation and colour
• Concern the extraction, encapsulation and/or antioxidant activity of a specific biological material without providing insight that could be applied to a similar but different material,
• Containing only chemical analyses of biological materials.