Development of a Janus nanofibrous patch with antibacterial and anti-oxidative properties for urethral regeneration.

IF 13.3 1区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Theranostics Pub Date : 2025-05-25 eCollection Date: 2025-01-01 DOI:10.7150/thno.112435
Wang Wang, Zesheng Chen, Ruoyu Li, Zhijun Zhou, Guanyi Wang, Xinjun Su, Weikang Hu, Zijian Wang, Xingyuan Xiao, Bing Li
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引用次数: 0

Abstract

Background: Urethral injury is the primary cause of urinary tract stenosis and hydronephrosis. Limited by the common drawbacks of autografts, the clinical treatment of urethral injury remains challenging. In recent years, biocompatible and biodegradable biomaterials (BBBs) are emerging as a potential substitute for autografts to upgrade the research paradigms of regenerative medicine. However, ideal BBBs for urethral regeneration have rarely been reported. Methods: A Janus nanofibrous PC/SMC patch composed of the outer layer of PLLA/CRRI-3 nanofibers and the inner layer of SF/MCe heterojunction nanofibers were first fabricated. Its antibacterial and antioxidant properties were assessed. After passing biosafety evaluation, the patch's efficacy in repairing urethral defects was evaluated using a rabbit model, with repair outcomes analyzed via histological staining. Results: PC/SMC patch not only inhibits bacterial proliferation and survival via the release of the antibacterial peptide CRRI-3, but it also relieves oxidation stress and promotes tissue regeneration by the nanozyme-like activities of the MCe heterojunction. The biocompatibility of PC/SMC patch has met the general requirements for Class-III medical devices. The application in vivo was evaluated using a urethral injury model of rabbits. The results showed that PC/SMC patch could improve urethral regeneration and prevent urethral stricture via multiple mechanisms, including promoting re-epithelialization, cell proliferation and M2 macrophage polarization, and inhibiting of fibrosis and scar formation. Conclusion: The PC/SMC nanofibrous patch has good biocompatibility and antibacterial properties, and can effectively promote the regeneration and repair of urethral tissue.

具有抗菌和抗氧化性能的Janus纳米纤维贴片用于尿道再生的研制。
背景:尿道损伤是泌尿道狭窄和肾积水的主要原因。尿道损伤的临床治疗受到自体尿道移植物常见缺陷的限制,仍然具有挑战性。近年来,生物相容性和可生物降解生物材料(bbb)作为一种潜在的自体移植物替代品正在兴起,以提升再生医学的研究范式。然而,用于尿道再生的理想血脑屏障鲜有报道。方法:首先制备由外层PLLA/CRRI-3纳米纤维和内层SF/MCe异质结纳米纤维组成的Janus纳米纤维PC/SMC贴片。对其抗菌和抗氧化性能进行了评价。通过生物安全性评价后,采用兔模型评价该贴片修复尿道缺损的效果,并通过组织学染色分析修复效果。结果:PC/SMC贴片不仅可以通过释放抗菌肽CRRI-3抑制细菌增殖和存活,还可以通过MCe异质结的纳米酶样活性缓解氧化应激,促进组织再生。PC/SMC贴片的生物相容性满足iii类医疗器械的一般要求。采用家兔尿道损伤模型对其在体内的应用进行了评价。结果表明,PC/SMC贴片可通过促进再上皮化、细胞增殖和M2巨噬细胞极化、抑制纤维化和瘢痕形成等多种机制促进尿道再生,预防尿道狭窄。结论:PC/SMC纳米纤维贴片具有良好的生物相容性和抗菌性能,能有效促进尿道组织的再生和修复。
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来源期刊
Theranostics
Theranostics MEDICINE, RESEARCH & EXPERIMENTAL-
CiteScore
25.40
自引率
1.60%
发文量
433
审稿时长
1 months
期刊介绍: Theranostics serves as a pivotal platform for the exchange of clinical and scientific insights within the diagnostic and therapeutic molecular and nanomedicine community, along with allied professions engaged in integrating molecular imaging and therapy. As a multidisciplinary journal, Theranostics showcases innovative research articles spanning fields such as in vitro diagnostics and prognostics, in vivo molecular imaging, molecular therapeutics, image-guided therapy, biosensor technology, nanobiosensors, bioelectronics, system biology, translational medicine, point-of-care applications, and personalized medicine. Encouraging a broad spectrum of biomedical research with potential theranostic applications, the journal rigorously peer-reviews primary research, alongside publishing reviews, news, and commentary that aim to bridge the gap between the laboratory, clinic, and biotechnology industries.
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