Proteomic analysis of hydrogen peroxide-treated human chondrocytes shows endoplasmic reticulum stress, cytoskeleton remodeling, and altered secretome composition.

Abstract

Background: Chondrocyte homeostasis is vital for maintaining the extracellular matrix (ECM) and overall cartilage health. In osteoarthritis (OA), for example, oxidative stress resulting from redox imbalances can disrupt chondrocyte homeostasis, leading to cartilage degradation. Hydrogen peroxide (H₂O₂), a reactive oxygen species (ROS), is a key mediator of oxidative stress and contributes to chondrocyte apoptosis and ECM degradation. Previous studies have explored individual protein responses to oxidative stress; however, a comprehensive proteomic analysis in chondrocytes has not been conducted. In this study, we aimed to assess the global proteomic alterations in chondrocytes exposed to H₂O₂ using a shotgun proteomics approach, which enables the detection of a broad spectrum of proteomic changes.

Methods: Chondrocytes were treated with H₂O₂ for 1, 4, and 16 h followed by protein extraction and processing, including denaturation, alkylation, and trypsin digestion. The peptides were then acidified, desalted, dried, and resuspended for LC-MS/MS. Proteomics data were analyzed using MaxQuant software to identify and quantify proteins. Secretome analysis was performed to examine protein secretion changes under oxidative stress. The statistical significance of all proteomics and secretome data was assessed using a two-tailed Student's t-test with a permutation-based FDR and an S0 parameter of 0.1 in the Perseus software. Other methods, including quantitative PCR, western blotting, and immunofluorescence, were employed to complement the proteomic analysis.

Results: Our findings revealed that oxidative stress primarily affected the endoplasmic reticulum (ER), causing notable alterations in the expression of ER-associated proteins, redox-responsive enzymes, chaperones, and sialyltransferases. These changes increased intracellular accumulation of ECM proteins and decreased secretion into the extracellular environment, indicating impaired protein trafficking and secretion. Additionally, immune-related pathways were activated in the long term, with a short-term upregulation of inflammatory markers, such as interleukin (IL)-6 and IL-18, although the levels of matrix metalloproteinases (MMPs) remained stable, indicating that not only complex inflammatory stimuli, but also oxidative stress responses can disrupt ECM homeostasis.

Conclusions: Our study demonstrates a detailed proteomic view of the stress response of H₂O_2-treated chondrocytes, highlighting the significant changes in ER function, cytoskeletal remodeling, protein secretion, and immune responses. These changes suggest that oxidative stress impacts ECM balance and can contribute to cartilage disorders, such as OA, through different mechanisms than what is usually observed with inflammatory stimulus, offering new insights into the molecular mechanisms underlying oxidative stress in chondrocytes.