Current biological, chemical and physical gene delivery approaches for producing induced pluripotent stem cells (iPSCs).

IF 4.2 3区医学 Q1 PHARMACOLOGY & PHARMACY

European journal of pharmacology Pub Date : 2025-06-11 DOI:10.1016/j.ejphar.2025.177786

Elahe Izadi, Seyedeh Shima Mohammad, Mazdak Ganjalikhani Hakemi, Shirin Eshghi, Leila Saremi, Zohreh Saltanatpour, Amir Ali Hamidieh

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引用次数: 0

Abstract

The discovery of induced pluripotent stem cells (iPSCs) has revolutionized the research platform for disease modeling, drug discovery, cell therapy and regenerative medicine. Due to the importance of iPSCs over the past decade, various studies have focused on finding a safe and efficient gene delivery system to introduce reprogramming factors (RFs) into somatic cells, particularly for clinical applications. However, generated iPSCs from any source must undergo genomic, epigenomic, and functional characterizations to ensure they are free of somatic memories and safe for clinical application. In this review, almost all the employed cargos for delivering RFs into somatic cells were investigated, focusing on biological, chemical, and physical approaches to promote reprogramming efficiency and reduce exogenous factors. Moreover, the advantages and disadvantages of each approach were highlighted and cutting-edge technologies in iPSCs technology were also discussed. This review aims to provide a comprehensive overview discussing how to improve the efficiency and quality of iPSCs production.

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目前用于诱导多能干细胞（iPSCs）的生物、化学和物理基因传递方法。

诱导多能干细胞（iPSCs）的发现彻底改变了疾病建模、药物发现、细胞治疗和再生医学的研究平台。由于iPSCs在过去十年中的重要性，各种研究都集中在寻找一种安全有效的基因传递系统，将重编程因子（RFs）引入体细胞，特别是用于临床应用。然而，从任何来源生成的iPSCs都必须经过基因组、表观基因组和功能表征，以确保它们没有体细胞记忆，可以安全用于临床应用。在这篇综述中，研究了几乎所有用于向体细胞输送射频信号的载体，重点介绍了生物、化学和物理途径，以提高重编程效率和减少外源因素。此外，还介绍了每种方法的优缺点，并讨论了iPSCs技术的前沿技术。本文就如何提高诱导多能干细胞的生产效率和质量作一综述。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European journal of pharmacology 医学-药学

CiteScore

9.00

自引率

0.00%

发文量

572

审稿时长

34 days

期刊介绍： The European Journal of Pharmacology publishes research papers covering all aspects of experimental pharmacology with focus on the mechanism of action of structurally identified compounds affecting biological systems. The scope includes: Behavioural pharmacology Neuropharmacology and analgesia Cardiovascular pharmacology Pulmonary, gastrointestinal and urogenital pharmacology Endocrine pharmacology Immunopharmacology and inflammation Molecular and cellular pharmacology Regenerative pharmacology Biologicals and biotherapeutics Translational pharmacology Nutriceutical pharmacology.