The effect of chrysin on ischemia-reperfusion injury in the rat epigastric artery skin island flap.

IF 1
Simay Ersahin, Nesrin Tan Baser, Narin Karagoz, Gulcin Simsek
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Abstract

Background: The return of oxygenated blood by reperfusion after ischemia triggers paradoxical tissue damage known as ischemia-reperfusion (I/R) injury. This study aims to investigate the effect of chrysin (C) on I/R injury in epigastric island flaps in rats.

Methods: Thirty male Wistar albino rats were randomly divided into five groups (n=6): the sham control group (Group I), the flap I/R-untreated group (Group II), the I/R + C 10 mg/kg/day group (Group III), the I/R + C 50 mg/kg/day group (Group IV), and the I/R + C 100 mg/kg/day (Group V). Chrysin was administered orally for seven days before and after surgery. Flap elevation was performed on the eighth day, followed by eight hours of induced ischemia. Flap survival rate, as well as biochemical and histopathological parameters, were evaluated.

Results: Oral administration of chrysin significantly reduced fibroblast activity at all treatment doses in rats subjected to I/R injury. Although improvements were observed in flap survival rate, oxidative stress index (OSI), and vascular proliferation at 10 mg/kg/day and 50 mg/kg/day doses, as well as in total oxidant status (TOS), tumor necrosis factor-alpha (TNF-α), active inflammation, chronic inflammation and ulceration across all treatment doses, these changes did not reach statistical significance. Total antioxidant capacity (TAC) values, consistent with existing literature, did not appear to influence the positive outcomes.

Conclusion: This study examined the effects of chrysin treatment on ischemia-reperfusion injury using a rat inferior epigastric artery skin island flap model. Although favorable molecular changes were observed, these did not translate into significant improvements in clinical outcomes.

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菊花素对大鼠胃上动脉岛状皮瓣缺血再灌注损伤的影响。
背景:缺血后氧合血通过再灌注返回会引发矛盾的组织损伤,称为缺血-再灌注(I/R)损伤。本研究旨在探讨菊花素(C)对大鼠上腹部岛状皮瓣I/R损伤的影响。方法:雄性Wistar白化大鼠30只,随机分为5组(n=6):假对照组(I组)、皮瓣I/R-未处理组(II组)、I/R + C 10 mg/kg/d组(III组)、I/R + C 50 mg/kg/d组(IV组)、I/R + C 100 mg/kg/d组(V组)。术前、术后7天口服菊花素。第8天皮瓣抬高,8小时诱导缺血。观察皮瓣存活率、生化指标及组织病理学指标。结果:在I/R损伤大鼠的所有治疗剂量下,口服菊花素均可显著降低成纤维细胞活性。虽然在10 mg/kg/天和50 mg/kg/天剂量下,皮瓣存活率、氧化应激指数(OSI)和血管增殖,以及总氧化状态(TOS)、肿瘤坏死因子-α (TNF-α)、活动性炎症、慢性炎症和溃疡均有改善,但这些变化没有达到统计学意义。总抗氧化能力(TAC)值与现有文献一致,似乎不影响阳性结果。结论:本研究采用大鼠腹壁下动脉岛状皮瓣模型,观察金菊素对缺血再灌注损伤的影响。虽然观察到有利的分子变化,但这些并没有转化为临床结果的显着改善。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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