Using a Live Analysis System to Study Amyloplast Replication in Arabidopsis Ovule Integuments.

IF 1 Q3 BIOLOGY
Makoto T Fujiwara, Rin Arakawa, Tomoko Abe, Ryuuichi D Itoh
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引用次数: 0

Abstract

Amyloplasts, non-photosynthetic plastids specialized for starch synthesis and storage, proliferate in storage tissue cells of plants. To date, studies of amyloplast replication in roots and the ovule nucelli from various plant species have been performed using electron and fluorescence microscopy. However, a complete understanding of amyloplast replication remains unclear due to the absence of experimental systems capable of tracking their morphology and behavior in living cells. Recently, we demonstrated that Arabidopsis ovule integument could provide a platform for live-cell imaging of amyloplast replication. This system enables precise analysis of amyloplast number and shape, including the behavior of stroma-filled tubules (stromules), during proplastid-to-amyloplast development in post-mitotic cells. Here, we provide technical guidelines for observing and quantifying amyloplasts using conventional fluorescence microscopy in wild-type and several plastid-division mutants of Arabidopsis. Key features • Novel approach for investigating amyloplast differentiation and replication in plant cells. • Detection of stroma-labeled amyloplasts in whole-mount ovules using conventional fluorescence microscopy. • Facilitates quantitative and comparative analysis of amyloplast proliferation using various Arabidopsis resources. • Enables high-resolution analysis of changing amyloplast and stromule morphologies in living cells.

利用活体分析系统研究拟南芥胚珠珠被淀粉质体复制。
淀粉质体是一种非光合质体,专门用于淀粉的合成和储存,在植物的储存组织细胞中增殖。迄今为止,已经使用电子和荧光显微镜对各种植物的根和胚珠核的淀粉质体复制进行了研究。然而,由于缺乏能够在活细胞中跟踪其形态和行为的实验系统,对淀粉质体复制的完整理解仍然不清楚。最近,我们证明了拟南芥胚珠被可以为淀粉体复制的活细胞成像提供一个平台。该系统能够精确分析有丝分裂后细胞从原质体到淀粉体发育过程中淀粉体的数量和形状,包括充满基质的小管(间质)的行为。在这里,我们提供了使用常规荧光显微镜观察和定量拟南芥野生型和几种质体分裂突变体的淀粉质体的技术指南。•研究植物细胞中淀粉体分化和复制的新方法。•使用常规荧光显微镜检测整个胚珠中基质标记的淀粉质体。•利用各种拟南芥资源促进淀粉质体增殖的定量和比较分析。•能够高分辨率分析活细胞中淀粉体和基质形态的变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
1.50
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0.00%
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