{"title":"Kinetics of recB-dependent repair: Relationship to post-UV inactivation of the prophage","authors":"Željko Trgovčević, Drago Petranović, Erika Salaj-Šmic, Mirjana Petranović","doi":"10.1016/0167-8817(87)90029-0","DOIUrl":null,"url":null,"abstract":"<div><p>By making use of the temperature-sensitive mutant <em>recB270</em>, we showed that the RecBCD enzyme is needed for repair between 1 and 4 h after UV exposure. <em>recB</em>-dependent prophage inactivation (Petranović et al. (1984), Mol. Gen. Genet., 196, 167–169) takes place in all dying cells during the same period of time. The kinetics of decrease in the yield of recombinants in phage-propage crosses resemble those of prophage inactivation in UV-irradiated bacteria. This indicates that recombination processes (including site-specific recombination required for prophage excision) are blocked in cells destined to die. On the basis of our results, we suggest that a large fraction of damaged cells is rescued by the RecA−RecBCD recombination pathway. If repair is unsuccessful, RecA−RecBCD recombination intermediated persist in the irradiated cells leading to prophage inactivation.</p></div>","PeriodicalId":100936,"journal":{"name":"Mutation Research/DNA Repair Reports","volume":"184 1","pages":"Pages 1-6"},"PeriodicalIF":0.0000,"publicationDate":"1987-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0167-8817(87)90029-0","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation Research/DNA Repair Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0167881787900290","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
By making use of the temperature-sensitive mutant recB270, we showed that the RecBCD enzyme is needed for repair between 1 and 4 h after UV exposure. recB-dependent prophage inactivation (Petranović et al. (1984), Mol. Gen. Genet., 196, 167–169) takes place in all dying cells during the same period of time. The kinetics of decrease in the yield of recombinants in phage-propage crosses resemble those of prophage inactivation in UV-irradiated bacteria. This indicates that recombination processes (including site-specific recombination required for prophage excision) are blocked in cells destined to die. On the basis of our results, we suggest that a large fraction of damaged cells is rescued by the RecA−RecBCD recombination pathway. If repair is unsuccessful, RecA−RecBCD recombination intermediated persist in the irradiated cells leading to prophage inactivation.
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