{"title":"Hyaluronidase Inhibitory Activity of Polysaccharides Separated from a Fermented Beverage of Plant Extracts.","authors":"Hideki Okada, Akira Yamamori, Naoki Kawazoe, Keiji Ueno, Shuichi Onodera","doi":"10.5458/jag.7202202","DOIUrl":null,"url":null,"abstract":"<p><p>Super Ohtaka<sup>®</sup>, a fermented beverage of plant extracts, is prepared from approximately 50 kinds of vegetables and fruits is a naturally fermented mainly by lactic acid bacteria (<i>Leuconostoc</i> spp.) and yeast (<i>Zygosaccharomyces</i> spp.). In this study, we separated water-soluble polysaccharides from Super Ohtaka<sup>®</sup> using dialysis and chromatography, yielding four polysaccharide fractions. The polysaccharide fraction designated as OEP3 exhibited hyaluronidase inhibitory activity. The half-maximal inhibitory concentration was 860 µg/mL. This polysaccharide not only stimulated macrophages but also inhibited hyaluronidase activity and showed weak 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity.</p>","PeriodicalId":14999,"journal":{"name":"Journal of applied glycoscience","volume":"72 2","pages":"7202202"},"PeriodicalIF":1.4000,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12149733/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of applied glycoscience","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5458/jag.7202202","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Super Ohtaka®, a fermented beverage of plant extracts, is prepared from approximately 50 kinds of vegetables and fruits is a naturally fermented mainly by lactic acid bacteria (Leuconostoc spp.) and yeast (Zygosaccharomyces spp.). In this study, we separated water-soluble polysaccharides from Super Ohtaka® using dialysis and chromatography, yielding four polysaccharide fractions. The polysaccharide fraction designated as OEP3 exhibited hyaluronidase inhibitory activity. The half-maximal inhibitory concentration was 860 µg/mL. This polysaccharide not only stimulated macrophages but also inhibited hyaluronidase activity and showed weak 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity.
Super Ohtaka®是一种植物提取物发酵饮料，由大约50种蔬菜和水果制成，主要由乳酸菌（Leuconostoc spp.）和酵母（Zygosaccharomyces spp.）自然发酵而成。在本研究中，我们用透析和色谱分离了Super Ohtaka®的水溶性多糖，得到了四个多糖部分。多糖部分命名为OEP3，显示出透明质酸酶抑制活性。半最大抑制浓度为860µg/mL。该多糖不仅能刺激巨噬细胞，还能抑制透明质酸酶活性，并表现出较弱的1,1-二苯基-2-苦酰肼自由基清除活性。
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