Correlation of Trace detection in Gene Xpert MTB/RIF ultra with MGIT TB culture in a high TB-endemic Country

IF 2.9 3区医学 Q3 IMMUNOLOGY

Tuberculosis Pub Date : 2025-06-09 DOI:10.1016/j.tube.2025.102666

Swapna Naik, Sweta Dhaneja, Archana Khilari, Shaoli Basu, Anjali Shetty, Camilla Rodrigues

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引用次数: 0

Abstract

Background

The Gene Xpert MTB/RIF Ultra is a Cartridge-Based Nucleic Acid Amplification Test (CBNAAT) for rapid detection of Mycobacterium tuberculosis (MTB) and rifampicin (RIF) drug susceptibility testing (DST) pattern.

Objective

1)To compare the culture results of samples detected as a Trace on Gene Xpert MTB/RIF Ultra with MTB growth in liquid culture. 2) To compare the rifampicin indeterminate results from Gene Xpert MTB/RIF Ultra with MGIT RIF DST pattern.

Materials and Methods

A retrospective case record study was conducted from January 1, 2020 to August 31, 2024. Samples were evaluated using Gene Xpert MTB/RIF Ultra and MGIT liquid culture. MTB isolates from MGIT liquid culture were subjected to MGIT RIF DST.

Result

A total of 1821 samples detected as trace in Gene Xpert MTB/RIF Ultra, 538 (29.54 %) grew in MGIT liquid culture, while 1283 (70.46 %) did not grow. Among the 538 Trace detected samples with RIF indeterminate results, a comparison with MGIT RIF DST revealed that 451 (83.83 %) were rifampicin sensitive, while 87 (16.17 %) were rifampicin resistance. 49 (9.10 %) of these were pulmonary samples, while 489 (90.90 %) were extrapulmonary samples.

Conclusion

Gene Xpert MTB/RIF Ultra is more rapid, more sensitive, less specific and more cost-effective compared to MGIT liquid culture.

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结核高发国家基因Xpert MTB/RIF ultra微量检测与MGIT结核培养的相关性

背景Xpert MTB/RIF Ultra基因是一种快速检测结核分枝杆菌（MTB）和利福平（RIF）药敏试验（DST）模式的核酸扩增试验（CBNAAT）。目的1)比较Xpert MTB/RIF Ultra基因痕量检测样品的培养结果与液体培养MTB的生长情况。2)比较Gene Xpert MTB/RIF Ultra与MGIT RIF DST模式的利福平不确定结果。材料与方法于2020年1月1日至2024年8月31日进行回顾性病例记录研究。使用Gene Xpert MTB/RIF Ultra和MGIT液体培养对样品进行评估。对MGIT液体培养的MTB分离株进行MGIT RIF DST检测。结果基因Xpert MTB/RIF Ultra检测到痕量样品1821份，其中538份（29.54%）在MGIT液体培养中生长，1283份（70.46%）未生长。在538份RIF结果不确定的痕量检测样品中，与MGIT RIF DST比较，451份（83.83%）对利福平敏感，87份（16.17%）对利福平耐药。肺标本49例（9.10%），肺外标本489例（90.90%）。结论与MGIT液体培养相比，gene Xpert MTB/RIF Ultra快速、灵敏、特异性低、性价比高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Tuberculosis 医学-呼吸系统

CiteScore

4.60

自引率

3.10%

发文量

审稿时长

49 days

期刊介绍： Tuberculosis is a speciality journal focusing on basic experimental research on tuberculosis, notably on bacteriological, immunological and pathogenesis aspects of the disease. The journal publishes original research and reviews on the host response and immunology of tuberculosis and the molecular biology, genetics and physiology of the organism, however discourages submissions with a meta-analytical focus (for example, articles based on searches of published articles in public electronic databases, especially where there is lack of evidence of the personal involvement of authors in the generation of such material). We do not publish Clinical Case-Studies. Areas on which submissions are welcomed include: -Clinical TrialsDiagnostics- Antimicrobial resistance- Immunology- Leprosy- Microbiology, including microbial physiology- Molecular epidemiology- Non-tuberculous Mycobacteria- Pathogenesis- Pathology- Vaccine development. This Journal does not accept case-reports. The resurgence of interest in tuberculosis has accelerated the pace of relevant research and Tuberculosis has grown with it, as the only journal dedicated to experimental biomedical research in tuberculosis.