Cheng Chen, Yang Meng, Yuxin Pan, Yang Zhang, Shaoyu He, Krishna Baral, Mingyi Zhao
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引用次数: 0
Abstract
Background: Ischemia-reperfusion (I/R) is an inevitable adverse outcome after heart transplantation, ultimately leading to graft dysfunction and affecting patient survival. Lysine β-hydroxybutyrylation (Kbhb) is a newly identified form of posttranslational modification that has been shown to be correlated with several cardiovascular diseases. This research sought to analyze the changes in Kbhb protein expression in myocardial tissues of mice with cold ischemia and reperfusion of the heart and to investigate its potential mechanisms.
Methods: The myocardial cold ischemia-reperfusion model was constructed using heterotopic abdominal heart transplantation in syngeneic C57/BL6J mice, and the myocardial tissue samples from 6 mice were examined using a combination of liquid chromatography-tandem mass spectrometry.
Results: After I/R, 43 upregulated and 18 downregulated proteins were identified. Among these, there were 50 upregulated and 18 downregulated Kbhb sites, including Ttn_K16192, Septin9_K355, and Auh_K186. Kyoto Encyclopedia of Genes and Genomes and protein-protein interaction network analyses indicated significant enrichment of differentially modified proteins in myocardial contraction and energy metabolism.
Conclusions: The differential expression of Kbhb-modified proteins revealed their potential roles in cold I/R injury after cardiac transplantation, laying the foundation for further exploration of the biological functions and clinical relevance.
期刊介绍：
The official journal of The Transplantation Society, and the International Liver Transplantation Society, Transplantation is published monthly and is the most cited and influential journal in the field, with more than 25,000 citations per year.
Transplantation has been the trusted source for extensive and timely coverage of the most important advances in transplantation for over 50 years. The Editors and Editorial Board are an international group of research and clinical leaders that includes many pioneers of the field, representing a diverse range of areas of expertise. This capable editorial team provides thoughtful and thorough peer review, and delivers rapid, careful and insightful editorial evaluation of all manuscripts submitted to the journal.
Transplantation is committed to rapid review and publication. The journal remains competitive with a time to first decision of fewer than 21 days. Transplantation was the first in the field to offer CME credit to its peer reviewers for reviews completed.
The journal publishes original research articles in original clinical science and original basic science. Short reports bring attention to research at the forefront of the field. Other areas covered include cell therapy and islet transplantation, immunobiology and genomics, and xenotransplantation.
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